Collaborative case study in the use of a new technology platform for fast and easy cell line development
In this case study, after flow sorting failed to generate a stable double positive cell population, even after multiple rounds of sorting, CellRaft Technology was able to identify and isolate rare double positive clones that formed viable colonies.
“The generation of these double positive clonal lines saved my lab of a lot of time and effort, allowing the rapid generation of multiple highly stable clones that had persistent expression for in vitro and in vivo assays. This proved superior to our flow-based methodologies, which were never as transgene positive or stable (in terms of expression) over multiple passages.”
Zachary Hartman, Ph.D., Director, Center for Applied Therapeutics. Associate Professor, Departments of Surgery, Pathology, and Immunology, Tumor Immunology and Immunotherapeutics Laboratory. Duke University. Review of the CellRaft Air System.