CASY Cell Counter & Analyzer

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Revolutionizing Cell Counting & Analysis with Precision and Efficiency

The CASY Cell Counter & Analyzer is at the forefront of cellular analysis technology, offering unparalleled precision and efficiency. Designed for the demanding needs of modern laboratories, this state-of-the-art instrument provides accurate, reliable, and rapid cell counting and analysis, enabling scientists to focus on groundbreaking research and results.

casy what do you like to count
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Benefits

When you seamlessly integrate CASY into your laboratory operations, you will experience a variety of benefits, including:

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Applications

The CASY Cell Counter & Analyzer is the perfect tool for a wide range of applications, including but not limited to:

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How it Works

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CASY counts not with a flat image, but with three dimensions – offering a true representation of life! CASY also counts cells using Electronic Current Exclusion (ECE) and Pulse Field Analysis to enable the accurate determination of viability without any influence by other variables such as staining or focusing. Viable cells with full cell volume are detected as the cell membrane provides a barrier for current, while dead cells with no membrane resistance have their nucleus detected.

CASY measures the conductivity between two electrodes separated by a defined pore. Cells are suspended in CASYton, a defined buffer solution. Cells pass through the pore and generate an electrical pulse that correlates with the volume of the cell with GMP/GLP compliant precision, allowing a maximum variation below ±2%.

casy stain free viability control

Aggregation – not a problem

As CASY determines the volume of each cell with a precision of ±2%, it does the same with aggregates. Unlike optical measurements, which either ignore aggregates or tries to draw some rings into rather low contrast images of them, CASY uses a mathematically correct, volume-based aggregation correction. Basically, CASY will see how often a single cell fits into each detected aggregate. Mean volume and size even indicate changes in the level of aggregation, for example with induced Pluripotent Stem cells (iPSC).
casy aggregation
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Customer Testimonials

"We study the regulation of cancer cell growth by signaling and metabolism. CASY is the ideal system for us to precisely measure proliferation and cell size of a wide range of cell lines. In this context, the new software makes instrument operation as well as data visualization and organization easy and convenient."
Dr. Wilhelm Palm
Head of the Junior Research Group Cell Signaling and Metabolism, German Cencer Resaerch Center
"We have successfully been using CASY for 10 years now. With CASY we are able to carry out reproducible cell culture experiments even when working with highly aggregated cells. CASY allows us to consider cell concentration and aggregation when seeding and harvesting cells, as both are measured by CASY. Measuring accurately cell number, the aggregation factor and the cell volume, gives us the opportunity to express analytical data as concentration per cell. Additionally and the molar amount of a substance within a cell can be calculated (used for example for metal uptake in µM)."
Prof. Dr. Julia Bornhorst
Food Chemistry, University of Wuppertal
"Cytotoxicity Assays using CASY enable us to perform fast, simple and reliable assessment of anti-cancer drug regimens and the subsequent chemosensitivity of carcinoma cell lines. A major advantage of CASY is that debris and dead cells can be masked in the analysis by a simple cell line specific setup and only viable cells and their aggregates are considered. However, as a complete cell status is recorded, also dead cells and debris data are available at any time."
Dr. J. Weinreich
Experimentelle Onkologie, Universiätsklinikum Tübingen
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Uses & Applications

Know your PBMCs

There are probably millions of PBMCs globally prepared from donor blood every day. The quality is however not only influenced by the selected separation media. It is very well known, that the composition also depends largely on the donor of the blood. The ratio between lymphocytes and monocytes can vary significantly between donors. Understanding these differences has a significant impact on the succes of the next steps performed with the PMBCs.

casy pbmcs transformed

The overlay shows how dramatically the total amount of lymphocytes and monocytes may vary from donor to donor. While the amount is balanced (red) in some cases, it may show a significantly higher amount of monocytes (blue) or lymphocytes (yellow).

Don’t miss HiMedia’s Lymphocytes separation Media (LSM) and Cryo Solutions for best results preparing and storing PBMCs.
Every time you measure your PBMCs using CASY Cell Counter & Analyzer you will have full information about the
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Track T cell activation

During T cell activation, cell proliferation and increases in volume are two critical parameters that CASY tracks very precisely at the same time. In the example below, human CD4 T Cells were stimulated with anti-CD3/CD28 Dynabeads.

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“Normal” cluster formation and cell size distribution during CD4 T cell stimulation. (Top) Cluster formation in the initial phase of activation of human CD4 T cells. (Bottom) CD4 T cells increased their cell size from 8 μm in freshly isolated quiescent T cells (green line) to 10 μm after 24 h (blue line) and finally to 12 μm after 48 h of stimulation (red line).

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Cluster formation and cell size development can change significantly under different metabolic conditions like the absence of glucose (A) or in the presence of lactic acid (B).

Whatever the impact, CASY will not only provide an accurate cell number, but you can also precisely analyze changes in volume.

iPSC - flexible handling, full control

CASY will change the way you work with induced pluripotent stem cells (iPSC). Like for other types of samples, no special sample preparation is required. In the case of iPSCs, this is truer than ever before. Choose trypsin, Accutase, EDTA or maybe another method – CASY will count your cells independent of the level of aggregation. Moreover, Mean Size and Mean Volume make the level of aggregation measurable.

So, stop torturing your cells while preparing them for other counting methods – start using CASY.

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Human iPSC have been prepared with Trypsin (orange), Accutase (yellow) and EDTA (red). The level of aggregation is visible easily. Volume based aggregation correction allows counting them with the same easy to use template and to provide robust accurate and reproducible results.

Primary Cells – not a challenge

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Overlay of human endothelial cells fresh isolated (orange) and after 1st passage (yellow)

Samples of primary cells often contain much debris, dead cells or even other cell types. CASY Cell Counter and Analyzer will help you to overcome issues, making counting easy. In the example shown, the measurement of freshly isolated human endothelial cells was compared with a measurement after the first passage. Even when the fresh material was containing much debris, dead cells and viable cells were much more heterogeneous, counting is not a problem and results are reliable and reproducible.

Insect cell / Baculovirus – stop before they burst

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During baculovirus infection the cell volume of SF9 cells changes significantly. Before infection (red) cells are about 15µm in diameter. During infection, cells (green) grow to sizes of 19µm or more.

CASY Counter and Analyzer is not only able to measure the change in volume. CASY also allows to standardize the process so that infection can be stopped before cells burst.

Erythrocytes and Platelets

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Erythrocytes are the most dominant cell type in fresh blood (orange) and show a peak at about 5.1µm in the case presented. Stills CASY can detect populations of active and inactive platelets in fresh blood. After centrifugation platelets remain (blue) and can be detected separately.

Saccharomyces cerevisiae

One of the most common commercial uses of Saccharomyces cerevisiae is brewing beer. Knowing the exact number of cells and their viability are two very critical parameters in the process, but not easy to obtain. Many breweries rely on CASY throughout the process.

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CASY will detect and the overlay can make the visible smallest changes. In this case, normal lager yeast (green) is compared to a sample which was obtained after a wild yeast infection (red).

If you are working with Candida albicans or Candida glabrata or something related, contact us to learn more or ask for a demo.

Algae – watch them bud

Raphidocelis subcapitata is a sweet water algae that is used in environmental testing. Growth and budding can be synchronized by light. CASY allows for precise following of the budding process.

R. subcapitata has a cell size of 6µm and each mother cell buds into 4 daughter cells. As budding begins, increasing numbers of single daughter cells appear. The budding continues over a time period of 4 to 5 hours, increasing the number of single buds. In parallel, the mother cells at 6µm almost completely disappear. 

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In some cases CASY can be used in an indirect way: In this lab, the amount of algae provided as food for Daphnia needed to be standardized. The appetite of Daphnia correlates with water quality. Therefore CASY was used to measure the initial amount of algae and what was left after one hour.

Cultivating bacteria in despair?

Bacteria grow rapidly and the cell number doubles often in 20 min or less. Counting them can be a real challenge and results are normally not precise or accurate.

Commonly used plating needs a long time until a result is at hand and the accuracy is very limited. One of the problems is that the number of bacteria counted often does not reflect a representative sample due to the high concentration of cells in culture. Alternative methods like measuring optical density (OD) only provide a rough idea of the concentration range.

If you are looking for a fast and precise way to count your bacteria, try CASY. Normally, within 45 seconds CASY will count up to 50,000 cells and provide a precise cell number.

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Besides E. coli (top) and B. subtillis a huge number of other different bacteria have already been successfully counted using CASY:

A Leptospira interrogans Salmonella thyphimurium
Acinetobacter baylyi M Serratia marcescens
Aeromonas salmonicida Malikia spinosa Shimwellia blattae
Alcaligenes faecalis Mycobacterium smegmatis Staphylococcus capitis
B N Staphylococcus aureus
Bacillus atrophaeus Neisseria canis Staphylococcus carnosus
Bacillus cereus P Staphylococcus epidermidis
Bacillus subtilis Pasteurella aerogenes Staphylococcus gallinarium
Bacteroides ovatus Pasteurella multocida Staphylococcus saprophyticus
Bordetella petrii Providencia heimbachae Staphylococcus simulans
C Prteus myxofaciens Staphylococcus xylosus
Comamonas acidovorans Pseudomonas aeruginosa Streptomyces spectabilis
Comamonas testosteronii Pseudomonas chloraphis Synechococcus elongatus
E Pseudomonas fluorescens Streptomyces spectabilis
Edwarsiella hoshinae Pseudomonas monteilii V
Enterobacter cloacae Pseudomonas mosselii Vibrio anguillarum
Erwinia aphidicola Pseudomonas pseudoalcaligenes Vibrio parahaemolyticus
Escherichia Coli Pseudomonas putida Y
K Pseudomonas stutzerii Yersina bercovieri
Klebsiella pneumoniae R Yersinia ruckeri
Kluyvera georgiana Rahnella aquatilis
L S
Lactobacillus Salmonella sp.

Fungi spores

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Ever wondered whether you have haploid and diploid spores?

Volume based size measurement is very precise and allows to discriminate between haploid (yellow) and diploid (orange) spores from Aspergillus niger. CASY measures many other spores too, just try it!

How to improve counting pollen

The answer is simple – use CASY.

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A complete workflow was recently published by Kakui et al. and is available for download. CASY provides precise counts for pollen. Even more, CASY can distinguish between pollen of different size or between fully- and undeveloped pollen.

Trypanosoma or Leishmania

How to measure something with a flagellum, like Leishmania?

CASY can do!

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CASY’s unique Pulse Field Area technology, detecting cells with 1MHZ while passing the pore, is the basis for the precise detection and exact volume-based size determination of elongated cells even if they carry a flagellum. Leishmania in promastigote stage is a good example and not a challenge for CASY.