CellRaft System for Inverted Microscopes
For scientists who do not have access to an AIR system, we offer an approach to use the CytoSort Array manually. We developed an accessory device that can fit a standard inverted microscope. Users can then use the microscope to view their cells on the arrays, manually release individual CellRafts, and manually collect/deposit those CellRafts into standard multiwell plates or PCR tubes.
Accessory Device for Inverted Microscopes
This device is designed to fit a range of microscope models with differing objective diameters. After fitting over the objective, the device is connected to a control unit. With the push of a button, the user actuates a needle to release an individual CellRaft from the CytoSort Array.
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Placement of CellRaft release device onto the microscope objective. (A) Release device and controller placed next to microscope. (B) Controller wires inserted from under microscope stage. (C) Release device attached to controller. (D) Release device (with cap) placed over microscope objective. (E) Release device placed over objective with cap removed. (F) Release device removed with tightening ring attached to microscope objective. (G) Release device reattached to tightening ring by pressing vertically down.