The CellRaft Array for use with standard inverted microscopes is currently available in two sizes – 12,000 microwells having a microwell dimension of 200 x 200 microns, or 44,000 microwells having a microwell dimension of 100 x 100 microns.
Each microwell contains a releasable CellRaft that both isolates the cells and allows individual recovery by using the Release Device. Cells placed on the CellRaft Array are localized on the CellRaft surface and remain viable. The entire array is housed in a plastic cassette similar to a Petri dish, making it familiar to the biological research community.
Once cells are adhered to the CellRafts, the CellRaft Array can be screened to identify target cells or colonies. The CellRaft Array can be imaged by brightfield, fluorescence and confocal microscopy. Individually released CellRafts can be transferred to analysis tubes for biochemical or molecular analysis using one of the Collection Devices
Match the CellRaft Array to your Specific Application
Single Reservoir – 100 micron CellRaft
Best choice for single cell isolation and recovery
Quad Reservoir – 100 micron CellRaft
Best choice for single cell isolation and recovery under differing culture media using a single cassette
Single Reservoir – 200 micron CellRaft
Best choice for clonal expansion and colony selection
Quad Reservoir – 200 micron CellRaft
The CellRaft System release device has been designed to fit a range of objective diameters and fits over a standard objective on your inverted microscope. The wire leads connect to the controller box. The controller box actuates the motor in the release device to provide a precise actuation distance to release individual CellRafts in the CellRaft Array.
Placement of CellRaft release device onto the microscope objective. (A) Release device and controller placed next to microscope. (B) Controller wires inserted from under microscope stage. (C) Release device attached to controller. (D) Release device (with cap) placed over microscope objective. (E) Release device placed over objective with cap removed. (F) Release device removed with tightening ring attached to microscope objective. (G) Release device reattached to tightening ring by pressing vertically down.
Each microwell of the CellRaft Array contains a CellRaft that can be individually released. The CellRafts contain paramagnetic particles and can be collected using a magnetic wand and then transferred to a collection plate. Cell Microsystems provides a variety of collection devices suitable for a range of product applications.
Collecting CellRafts with the Standard Wand and Transferring to a Collection Plate fitted with a 96-well plate. (A) Multiwell Collection Plate. (B) Multiwell Collection Plate containing a 96 well dish. (C) CellRaft Array with Standard Wand. (D) Standard Wand held over the center of the Multiwell Collection Plate. (E) Standard Wand lowered into a well in the 96 well dish to transfer a CellRaft.
Collecting CellRafts with the MiniWand and Transferring to a PCR Tube. (A) PCR Collection Plate. (B) MiniWand inserted into media to collect a released CellRaft (C) CellRaft transferred from a MiniWand to a PCR tube over the PCR Collection Plate.