The CytoSort Array may be used with standard inverted microscopes fitted with the CellRaft Release Device. Magnetic wands and collection plates are also available for collecting CellRafts into standard multiwell plates or PCR tubes.
Release Device for Inverted Microscopes
The Release Device has been designed to fit a range of objective diameters and fits over a standard objective on your inverted microscope. The wire leads connect to the controller box. The controller box actuates the motor in the release device to provide a precise actuation distance to release individual CellRafts in the CytoSort Array.
Placement of CellRaft release device onto the microscope objective. (A) Release device and controller placed next to microscope. (B) Controller wires inserted from under microscope stage. (C) Release device attached to controller. (D) Release device (with cap) placed over microscope objective. (E) Release device placed over objective with cap removed. (F) Release device removed with tightening ring attached to microscope objective. (G) Release device reattached to tightening ring by pressing vertically down.
Magnetic Wands and Collection Plates
Each microwell of the CytoSort Array contains a CellRaft that can be individually released. The CellRafts contain paramagnetic particles and can be collected using a magnetic wand and then transferred to a collection plate. Cell Microsystems provides a variety of collection devices suitable for a range of product applications.
Collecting CellRafts with the Standard Wand and Transferring to a Collection Plate fitted with a 96-well plate. (A) Multiwell Collection Plate. (B) Multiwell Collection Plate containing a 96 well dish. (C) CytoSort Array with Standard Wand. (D) Standard Wand held over the center of the Multiwell Collection Plate. (E) Standard Wand lowered into a well in the 96 well dish to transfer a CellRaft.
Collecting CellRafts with the MiniWand and Transferring to a PCR Tube. (A) PCR Collection Plate. (B) MiniWand inserted into media to collect a released CellRaft (C) CellRaft transferred from a MiniWand to a PCR tube over the PCR Collection Plate.