Here are a few ways CellRaft® Technology is being used in customer labs.
Scientific Poster: Accelerating Generation of Single Cell Clones
Poster: Leveraging a CRISPR-based LAPSE Workflow to Guide Drug Targeting Strategies
Case Study: Identification of a Rare Double Positive Clone
Max Planck Institute
Journal Article: Detection of unintended on-target effects in CRISPR genome editing by DNA donors carrying diagnostic substitutions
Journal Article: A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A
Washington University School of Medicine, St Louis, MO
Journal Article: Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations
University of North Carolina Chapel Hill
Journal Article: PDS5A and PDS5B differentially affect gene expression without altering cohesin localization across the genome
Memorial Sloan Kettering Cancer Center
Journal Article: RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells
Quotes from our customers
Albert Einstein College of Medicine
Rating 5.0 / review on SelectScience: "I can recommend it to anyone who wants to pick single cells or clone... It is user-friendly."
Reviewer: Moonsook Lee. Application Area: Whole genome amplification from single cells. Review Date: 13 Dec 2022 | CellRaft AIR System.
Rating Extremely Satisfied (5 Stars): “The system is great for difficult-to-clone cell lines and has powerful imaging technology to help with our cell engineering assays.”
Jillian Pattison, Senior Scientific Researcher.
Lineberger Comprehensive Cancer Center / The University of North Carolina at Chapel Hill
“We recommend the CellRaft Technology for our colleagues struggling with single cell isolation. Even with help from FACS, although single cell clones could be obtained, from our experiences, CellRaft not only speeds up this process by providing many more clones but more importantly, the unique monitoring process provided by CellRaft supplies additional information for every single clone with a higher quality of clones.”
Pengda Liu, Ph.D., Associate Professor of Biochemistry and Biophysics.
School of Medicine / The University of North Carolina at Chapel Hill
“We can now easily identify thousands of single organoids with various morphologies (size, shape, complexity) and isolate hundreds in less than an hour. We also use the CellRaft AIR System for cloning of transgenic or CRISPR-edited human intestinal stem cells. Prior to the air system, the efficiency of isolating clonal lines was very inefficient because of the low throughput. Now we can isolate hundreds or thousands of clonal organoids in an unprecedented short amount of time compared to before.”
See blog: Accelerating Organoid Development.
Scott Magness, Ph.D., Associate Professor, University of North Carolina – Chapel Hill, NC State University Joint Departments of Biomedical Engineering, UNC Departments of Medicine, Cell Biology, & Physiology Associate Professor of Biochemistry and Biophysics.
“The generation of these double positive clonal lines saved my lab of a lot of time and effort, allowing the rapid generation of multiple highly stable clones that had persistent expression for in vitro and in vivo assays. This proved superior to our flow-based methodologies, which were never as transgene positive or stable (in terms of expression) over multiple passages.”
Zachary Hartman, Ph.D., Director, Center for Applied Therapeutics. Associate Professor, Departments of Surgery, Pathology, and Immunology, Tumor Immunology and Immunotherapeutics Laboratory.