Migration & Invasion

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Cellular migration is an important biological process that occurs in both in health and disease as a part of wound healing, development, differentiation, inflammation, immune response, angiogenesis and metastasis. Chemical messengers are integral to most migration events and serve to direct cells to tissues or the vasculature via chemotaxis. Chemotaxis is a dynamic event with numerous steps of cell communication and response. In order to examine these processes in detail over time, it is necessary to observe cells in situ.

The BioFlux system can be used for angiogenesis assays with endothelial cells, screening different types of tumor cells for invasive phenotypes, screening compounds to inhibit or otherwise affect invasion and or angiogenesis, or to study chemotaxis of circulating cells in response to stimuli or inhibition.

After 36 hours, cells WGA (green) was used to stain cells and gel for 15 min post-flow. Nuclei are stained as well with Hoescht 33342 (blue). Individual cells were observed invading the gel matrix under conditions of Matrigel alone, VEGF, bFGF. Sprouting and process formation, which are early steps of angiogenesis, were noted under these conditions. The addition of fumagilin, a angiogenesis inhibitor, abrogated the invasion and the cells multiplied outside of the gel.

Key BioFlux Advantages For Migration & Invasion Studies

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Chemotactic Migration

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Using the 24 well dual flow plates, fMLP was perfused in inlet A (top) and media was perfused in inlet B (bottom). HL60 cells migrated towards the fMLP.