Sodium Channels

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Responsible for the rise of the action potential in neurons and cardiac muscle, sodium channels are important targets for drug research. Inhibitory drug interactions are investigated in safety pharmacology, pain research, and naturally-occurring toxin research. Although automated patch clamp systems have successfully studied sodium channels, many complicated assays stay elusive.

IonFlux Mercury can act as the missing link between HTS systems and the flexibility of manual patch clamp. 

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Nav 1.8 Lidocaine dose response
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Nav 1.7 lidocaine dose response
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Gigaseal single cell recordings of Nav 1.7

Sodium channel IonFlux assays benefit from:

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Paper
Research Spotlight

An Advanced Automated Patch Clamp Protocol Design to Investigate Drug-Ion Channel Binding Dynamics

Traditional screening projects that utilize automated patch-clamp instruments often overlook crucial details about how drugs work, such as the dynamics of binding and unbinding, as well as the modulation of gating. This study aims to showcase that a more comprehensive analysis can be achieved without sacrificing throughput. By utilizing the cutting-edge microfluidics-based automated patch clamp, IonFlux Mercury, this customer developed a rapid assessment method to understand the mechanism of action of sodium channel inhibitors, including their kinetics of association and dissociation in different states.