Mutant Screens

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Spontaneous bacterial and fungal mutations can be the beginning of disastrous conditions for both nosocomial- and community-derived infections. These spontaneous mutants have the potential to quickly become resistant to antibacterial and antifungal medications, forming biofilms much faster than wildtype bacteria and fungi. On the other hand, induced mutants via genetic engineering and recombination allows bacteria to be manipulated, producing beneficial results.

The BioFlux system allows for analysis of these mutants. The visualization and manipulation of the microbiological mutants paves way for new discoveries in the fields of pharmacology, biotechnology, medicine, and more.

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In this experiment, the BioFlux was used for analysis of methicillin-resistant Staphylococcus aureus (MRSA)-USA300 biofilm growths. Mutants show clear differences in growth under these conditions (b), as quantified via fluorescence (a) (Malhotra-Kumar et al., 2015).
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Hyphae formation in Candida albicans mutants was analyzed with a BioFlux imaging system (Mitchell et al., 2012).

Key BioFlux Advantages For Mutant Screen Studies