Tired of being limited by limiting dilution?
Traditionally, a limiting dilution method is used to isolate and generate single cell-derived clones, however, it is both time and cost prohibitive due to low efficiency of success and the manual labor input required.
CellRaft® Technology offers an affordable, automated solution to an otherwise labor-intensive workflow while:
- maintaining cell health
- providing time-course imaging for clonal verification
- delivering automated isolation of cells, colonies, or organoids
Grow More Colonies
Single cells are grown in a flask-like environment, without physically separating them, eliminating perturbation to cell physiology and ensuring the viability and vitality of single cells as they develop into clones. The CellRaft AIR® system is able to scan more than 60,000 individual cells per CellRaft Array, which is 500X more cells than a traditional 96-well plate with limiting dilution.
Experimental and cost analyses including hands-on time to run the protocol between limiting dilution and CellRaft Technology indicate that the latter delivers a high return on investment in terms of outgrowth efficiency, time, and cost.
Save Time and Money – Reduce the Cost per Clone
Finally! Image-based Proof of Monoclonality
Time-course imaging is a key feature of the CellRaft AIR System. After cells are seeded on the CellRaft Array, the array is serially scanned at the desired frequency to capture complete growth records of clonal development.
Challenges with Limiting Dilution:
- Lack of viable clones
- Lack of proof of monoclonality
- Requires significant lab supplies and equipment
- Hands-on time requirements
- Limited throughput
