No More Limiting Dilution

vline gray

Tired of Being Limited by Limiting Dilution?

Traditionally, a limiting dilution method is used to isolate and generate single cell-derived clones, however, it is both time and cost prohibitive due to low efficiency of success and the manual labor input required.
CellRaft® Technology offers an affordableautomated solution to an otherwise labor-intensive workflow while:
vline gray

Grow More Colonies

Single cells are grown in a flask-like environment, without physically separating them, eliminating perturbation to cell physiology and ensuring the viability and vitality of single cells as they develop into clones. The CellRaft AIR® system is able to scan more than 40,000 individual cells per CellRaft Array, which is 500X more cells than a traditional 96-well plate with limiting dilution.

Limiting Dilution vs. CellRaft Technology
Confluent colonies
(>50% confluent 10 days post-seeding)
vline gray

Save Time and Money – Reduce the Cost per Clone

Experimental and cost analyses including hands-on time to run the protocol between limiting dilution and CellRaft Technology indicate that the latter delivers a high return on investment in terms of outgrowth efficiency, time, and cost.

FInal Table for LD Graph 1
vline gray

Compare Limiting Dilution to CellRaft Technology

LD Comparison Chart 1
vline gray

Finally! Image-based Proof of Monoclonality

Time-course imaging is a key feature of the CellRaft AIR System. After cells are seeded on the CellRaft Array, the array is serially scanned at the desired frequency to capture complete growth records of clonal development.
Time Course Imaging