Automated Collection of Cells
Describes an automated method and system to isolate and collect cells using computerized image analysis of images of cells and their surroundings obtained from a digital imaging device or system. The system makes use of a “microwell array,” which can comprise a formed, elastomeric grid of indentations or “wells.” Many, most, or all of the wells in a microwell array can contain a releasable, microfabricated element, which can be referred to as a “raft.” A system and method is provided for cell collection that includes computerized identification and collection of rafts with isolated single cells or a specific group or groups of cells, eliminating the need for continuous human identification and selection.
Array of Micro-Elements for High Resolution and High Content Imaging and Sorting of Cells
An array of magnetic or paramagnetic micro-elements comprised of polysilsesquioxane is described having ultra-lowautofluorescence and other optical properties to improve microscopic imaging of cells or other object present on the array. These materials are also amenable to chemical modification allowing surface attachment of affinity capture moieties or chemical reporters for selective binding or analysis of cells, macromolecules or other targets.
Dissolution Guided Wetting of Structured Surfaces
Describes a process of preventing bubble formation in structured surfaces using either hydrophilic or hydrophobic sacrificial layers, depending on the application. Also includes microfabricated devices that can be wetted without bubble formation.
Array of Micromolded Structures for Sorting Adherent Cell
Describes an apparatus for collecting or culturing cells or cell colonies comprising a microwell array formed from an elastomeric material and having a plurality of rigid cell carriers releasably connected within each well, along with methods of use and a device for releasing the cell carriers.