One of the main goals in recombinant protein development is the establishment of high-quality monoclonal cell lines that consistently express large amounts of the given protein. Chinese hamster ovary (CHO) cell lines have dominated the industry as commercial hosts for recombinant protein production; however, the process of generating a homogenous CHO cell line is not trivial.
In this Raftnote, we compared the traditional limiting dilution method with the CellRaft® Array using the CellRaft AIR® System, evaluating both methods for single cell seeding efficiency, cloning efficiency, and clonal outgrowth.
