Comparison of Limiting Dilution and CellRaft® Technology for Cell Line Development

One of the main goals in recombinant protein development is the establishment of high-quality monoclonal cell lines that consistently express large amounts of the given protein. Chinese hamster ovary (CHO) cell lines have dominated the industry as commercial hosts for recombinant protein production; however, the process of generating a homogenous CHO cell line is not trivial.

In this Raftnote, we compared the traditional limiting dilution method with the CellRaft® Array using the CellRaft AIR® System, evaluating both methods for single cell seeding efficiency, cloning efficiency, and clonal outgrowth.

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