Cell Line Development using the CellRaft AIR® System

Therapeutic proteins play an essential role in the biological pharmaceutical market and are used in the treatment of many diseases, such as diabetes, cancer, and anemia. One of the main goals in recombinant protein development is the establishment of high-quality monoclonal cell lines that consistently express large amounts of the given protein. Chinese hamster ovary (CHO) cell lines have dominated the industry as commercial hosts for recombinant protein production; however, the process of generating a homogenous CHO cell line is not trivial.

The CellRaft technology developed by Cell Microsystems allows for streamlined and efficient cell line development by leveraging the combined power of our CytoSort Array cell culture consumable with our automated isolation and imaging instrument, the CellRaft AIR System.  The AIR System offers an automated solution to an otherwise labor-intensive workflow and supports cell health, time-course imaging for clonal verification, and automated isolation for downstream propagation, altogether providing an alternative that is more time, labor, and cost efficient for cell line development.

To demonstrate the value of the CellRaft technology for use in cell line development, we have compared the traditional limiting dilution method with the CytoSort® Array using the CellRaft AIR System, evaluating both methods for single cell seeding efficiency, cloning efficiency, and clonal outgrowth.