Cell Line Development – Comparing Limiting Dilution to the CellRaft AIR® System

One of the main goals in recombinant protein development is the establishment of high-quality monoclonal cell lines that consistently express large amounts of the given protein. Chinese hamster ovary (CHO) cell lines have dominated the industry as commercial hosts for recombinant protein production; however, the process of generating a homogenous CHO cell line is not trivial.

In this Raftnote, we compared the traditional limiting dilution method with the CellRaft Array using the CellRaft AIR System, evaluating both methods for single cell seeding efficiency, cloning efficiency, and clonal outgrowth.

Learn more about the CellRaft AIR® System