Imaging and Sorting Living Cells Stained with Vital Dyes on Cell Microsystems’ CytoSort Array and Automated CellRaft AIR System

Sorting and isolating single cells is a key sample preparation step in many contemporary workflows including genome editing by CRISPR/Cas9, single cell genomics and differentiation of induced pluripotent stem cells (IPSCs) along with a broad range of other methods. Cell Microsystems has developed the CellRaft AIR™ System to allow imaging-activated cell sorting and isolation of single living cells prior to these downstream analyses. Here, we present optimized methods for staining live cells on the Cell Microsystems CytoSort Array, a microwell array with releasable single cell isolation features called CellRafts. We also describe the use of the automated, bench-top AIR™ System for imaging and isolating vital dye-stained single cells using the CellRaft Technology.