Using the CellRaft AIR® System to Image, Identify, and Isolate Suspension Cells

The AIR System offers unique advantages over technologies, such as flow cytometry and droplet dispensers, designed to assess cells in suspension. The limitations to other technologies include impacts on cell viability, inability to interrogate small numbers of cells, and reliance on fluorescent markers or staining for cell characterization. To demonstrate the value of the CellRaft technology for use in suspension cell line development, we have established methodologies for attaching suspension cells to the CytoSort Arrays during single cell expansion while still allowing the cells to expand in suspension after the clone of interest has been isolated.