Know exactly what cells are in your library
Even in the best sorters, your enriched cell population often contains dead cells, the wrong cells, doublets, debris, or other artifacts. The imaging capabilities of the CellRaft AIR System provides the user with Day 0 scans to not only see the quality and phenotype of the cells, but to choose only the best cells.
Fast, effective and gentle automated isolation helps maintain native expression profiles. Selected cells are moved into lysis buffer quickly and gently with no need for trypsin, so phenotypes remain unperturbed, and quality remains high. No minimum required input and gentle movement allows the use of many cell types:
- Purified nucleus
Connect Phenotype to Genotype
The CellRaft AIR System’s unique design allows users to connect downstream nucleic and protein profiles to a specific individual cell, providing greater value to each experiment.
- Functional Genomics
- Transcriptomics and scRNA-seq
- Whole-genome Amplification
- Single Nucleus Sequencing (SNS)
- Copy Number Variation (CNV)
- Single Nucleotide Variation
Accelerate the Path to Discovery
How It Works
How It Works
Cells are plated on a CellRaft® Array using standard plating techniques, and eliminating the need for trypsin, scraping, high pressure fluidics, and limiting dilution. The array is scanned, imaged, and recorded, allowing the user to track and refer back to cells over time. Detailed images enable users to view subcellular features and truly distinguish cells of interest to perform a more sophisticated single cell isolation.
Target cells grow unperturbed in optimized conditions in the imaging platform, increasing cell viability, and downstream read depth. The CellRaft Quad and HexaQuad Arrays allow different media, compound dosing, and growth conditions to be tested on a single consumable.
When target cells are ready for collection, the gentle release mechanism leaves cells unperturbed, so transfer to a PCR or culture plate won’t affect the quality of the cells for downstream analysis. Cells are also moved into lysis buffer quickly, preventing the degradation of genetic material in sensitive cell lines.