Genomics

Know exactly what is going in your library

Quality downstream analysis starts with quality cells. 

Whether hand-picked or automatically selected, the CellRaft AIR System keeps a complete record of your cell collection, so you know you are getting the best cells:

  • Limit multiplets, debris, unwanted cells, and damaged or dying cells
  • Confirm viability upfront
  • See the cell phenotypes you are picking

  

 

Flexibility for a number of genomic analyses:

  • Functional Genomics
  • Transcriptomics and scRNA-seq
  • Whole-genome Amplification
  • Single Nucleus Sequencing (SNS)
  • Copy Number Variation (CNV)
  • Single Nucleotide Variation

 

Click here to learn tips and methods of these techniques with the CellRaft AIR System

 

Fast, effective and gentle to maintain native expression profiles. Selected cells are moved into lysis buffer quickly and gently with no need for trypsin, so phenotypes remain unperturbed and quality remains high.  No minimum required input and gentle movement allows the use many cell types:

  • Primary 
  • Stem
  • Genome-edited 
  • Purified nucleus

 

Click here to see publications using the CellRaft AIR System

 

How It Works

Even in the best sorters, your enriched cell population often contains dead cells, the wrong cells, doublets, debris, or other artifacts.  The imaging capabilities of the CellRaft AIR System provides the user with Day 0 scans to not only see the quality and phenotype of the cells, but to choose only the best cells.

Cells are plated on a CytoSort Array using standard plating techniques, and eliminating the need for trypsin, scraping, high pressure fluidics, and limiting dilution.  The array is scanned, imaged, and recorded, allowing the user to track and, refer back to, cells over time.  Detailed images enable users to view subcellular features and truly distinguish cells of interest to perform a more sophisticated single cell isolation.

Target cells grow unperturbed in optimized conditions in the imaging platform, increasing cell viability, and downstream read depth.  The CytoSort Quad and HexaQuad Arrays allow different media, compound dosing, and growth conditions to be tested on a single consumable.

When target cells are ready for collection, the gentle release mechanism leaves cells unperturbed, so transfer to a PCR or culture plate won’t affect the quality of the cells for downstream analysis.  Cells are also moved into lysis buffer quickly, preventing the degradation of genetic material in sensitive cell lines.