Introducing The CellRaft AIR® System with 3D capabilities
Launching Spring 2022
An expansion of Cell Microsystems’ core product, the CellRaft AIR® System, with CytoSort® Arrays and software optimized for 3D structures.
The CellRaft AIR System with 3D capabilities is the next evolution of tools for upstream characterization, enabling better knowledge of downstream data collection.
By deploying the AIR System workflow early in the discovery pipeline, users may eliminate non-viable compounds sooner than traditional methods, avoiding time-consuming manual manipulation, and saving resources by reducing consumable and media use while accelerating discovery.
A novel system that images, identifies, and isolates organoids.
In typical organoid cultures, the structures overlap, requiring multifocal imaging with expensive equipment. They grow at different sizes and rates, leading to heterogeneity in viability and phenotype, resulting in confusing and uninterpretable data. Organoids grown in bulk culture are stuck in viscous extracellular matrix and are difficult to retrieve intact.
The AIR System with 3D capabilities offers a solution with a fully automated imaging and isolation workflow for organoid culture.
Capture high-resolution images in brightfield and fluorescence throughout the full height of the organoid with z-stack imaging.
The images showing detailed phenotypic characterization are cataloged and easily exported for the desired use.
Our user-friendly proprietary software enables a one-touch full-array image scan
Brightfield Imaging of Mouse Hepatic Organoids
EpCAM & Hoechst Imaging of Mouse Hepatic Organoids
Identify CellRafts containing 3D structures of interest using CellRaft Cytometry™ to detect organoids. Visually verify single cell clonality, morphological data, and growth rate, critical data points for workflows requiring track and traceability to confirm clonality. Identified organoids can be further evaluated and selected for phenotypic characteristics, such as organoid diameter, circularity, and fluorescence intensity.
Perform phenotypic characterization with Raft Cytometry.
Identify and verify CellRafts containing 3D structures of interest and create sets of sub-populations for isolation
The Air System with 3D capabilities enables automated isolation of live, intact organoids without fixation, dissociation, or damage to the 3D structure. Organoids remain viable through isolation and continue to grow off-array.
Clonally-derived Organoid on a CellRaft®
Organoid is isolated on the CellRaft
Day 1 Organoid Growth
Day 5 Organoid Growth
3D CytoSort Array
Grow hundred of segregated organoids per array on the 3D CytoSort Array. Large surface areas allow cell growth up to 1mm in diameter on optically transparent, releasable polystyrene CellRafts.
Size: 500×500 micron microwells
CellRafts®: 2,114 per array