Stem Cells

A better way to handle sensitive cells

Providing an ideal approach to gently isolate single iPSCs or hESCs and generate precious cell lines. The CellRaft AIR System can eliminate expensive additives and provide greater yields to accelerate the path to new therapies.

More Cells, Less Work

  • Generate 100s of monoclonal iPSC lines.
  • Grow iPSCs in 2D or 3D culture.
  • Fully automate gentle isolation of viable, validated iPSC cell lines
  • Validate single cell from cell seeding 
  • Reduce cellular perturbations and spontaneous differentiation
  • Avoid limited dilution series
  • Decrease required hands-on time
  • Use ONE consumable

Increase Cell Viability

Cells share and enrich a common culture media while remaining singular and isolated.  This contiguous media approach is much more favorable and dramatically increases cell viability*. 

Single cells and colonies are isolated from the CellRaft Array without the need for trypsinization, resulting in native phenotypes and greater viability for downstream analysis.

*compared to FACS into a 96-well culture plate

How It Works

Stem cells are notoriously difficult to handle.  They don’t like to be alone and are quick to react to any perturbations.  With the CellRaft AIR® System, cells plated on the CellRaft® Array share a common culture media, allowing them to continue to signal each other and share kinases and growth factors.

The array is placed and stored in the AIR Imaging platform to limit disturbances.  The array is screened and undifferentiated single cell targets identified from Day 0.  Individual cells can grow and expand to the desired colony size in the CellRaft Array.  Colony growth is visually tracked using the imaging capabilities of the AIR System, allowing the user to easily track back to ensure clonality.  This limits the amount of array movement and hands-on time.

Target colonies are gently released from the CellRaft Array and moved to a standard size collection plate for further expansion and downstream analysis.

Click for Stem Cells Publications

Scientific Poster: Accelerating the Use of iPSCs in Personalized Medicine and Drug Discovery

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