Webinars

Cell Microsystems presents webinars featuring the CellRaft AIR® technology and applications to research, including CRISPR, genome editing, and single cell sorting and analysis.

A roadmap to hundreds of characterized suspension cell clones using a single consumable

01/10/2022
Jessica Hartman, Ph.D – Director of Product Application, Cell Microsytems

The most common question we receive about the AIR System is “Can we use suspension cells?”

While the AIR System excels at manipulating adherent cell types, suspension cells are vital for the success of a variety of biopharmaceutical applications, including large scale recombinant protein, vaccine, and monoclonal antibody production.

The AIR® System offers unique advantages over technologies, such as flow cytometry and droplet dispensers, designed to assess cells in suspension.

View this webinar to find out how your suspension cell applications can be adapted to the CellRaft AIR system to save you time and effort and get you the clones you need faster.

 

New methods for CRISPR cell line development and screening using the CellRaft AIR® System for automated sorting and cloning

William Buchser, PhD – Assistant Professor, Department of Genetics, Washington University in St. Louis, School of Medicine
Jacquelyn DuVall, PhD – Lead for Cellular and Genomics Applications, Cell Microsystems

Cloning cells after gene editing is a labor-intensive process due to issues around cell viability, clonality and overall cell culture efficiency. The CellRaft AIR System provides an imaging-based workflow which supports single cell viability and allows independent clonal colony growth in a matter of days instead of months. The System also supports imaging-based phenotyping prior to isolation of single cells and clonal colonies for CRISPR screening applications.

Watch the webinar on demand here:

Culture, Imaging and Isolation of Cells and Colonies using the CytoSort® Array with the AIR® System

Dr. Jacquelyn DuVall, Cell Microsystems, Inc.

The CellRaft Technology is a straightforward means of culturing, sorting and isolating single cells and clonal colonies. The product relies on a single-use consumable, the CytoSort Array, a microwell cell culture dish that allows single cells to be individually analyzed and retrieved for downstream culture and analysis. At this webinar, Cell Microsystem’s Jacquelyn DuVall, PhD will review protocols recommended by Cell Microsystems for the most efficient use of the CytoSort Array. Optimized practices for cell seeding, staining and applying coatings will be presented. Finally, the use of the benchtop AIR®; System, a fully integrated instrument which supports imaging and isolation of cells with the CytoSort Array will be described.

Click Here for the webinar slide deck.

Or, watch the webinar here:

CRISPR/Cas9 Genome Editing using the CellRaft AIR® System

Dr. Nick Trotta, Cell Microsystems, Inc.

In the webinar, Nick Trotta, Director of Product Applications and Market Development at Cell Microsystems, will discuss methods for using the CellRaft® Technology for gene editing, including:
• Imaging-based sorting of transfection-positive cells
• Colony growth and monitoring on the CytoSort® Array
• “Dynamic collection” of clones at several time points from a single cell suspension from a single CytoSort Array
Comparison to FACS for isolation of clones and efficiency of colony survival

Click Here for the webinar slide deck.

Or, watch the webinar here:

Single Nucleus Sequencing Enabled by the CellRaft® Technology

Dr. Mike McConnell, University of Virginia

Generating single cell genomics data from primary human samples for translational research is very challenging given that samples must be fresh (to preserve nucleic acid integrity) and large (to accommodate sorting and preparation by FACS). These challenges are uniquely eliminated by combining: 1) newly optimized methods for single nucleus isolation from cryopreserved tissues and 2) the CellRaft technology which prepares and sorts samples of any size.

This webinar summarizes recent sample preparation advances for CellRaft-based isolation of nuclei for downstream genomic analysis. Mike McConnell, PhD of the University of Virginia has pioneered several methods in single nucleus genomic analysis and will share his optimized methods for preparing nuclei for genomic analysis using the CellRaft Technology.

Click Here for the webinar slide deck.

Or, watch the webinar here:

Upcoming Events

SLAS 2022

Feb 5-9, 2022