TIGR Tissue Grinder and Dissociator

vline gray

Benefits

Traditional tissue dissociation involves a time-consuming, low-throughput enzymatic digestion and manual cutting of samples. This method often results in low yield with the increased risk of contamination or altering the integrity of the cells.  The TIGR Tissue Grinder and Dissociator overcomes these limitations:

tigr device logo on 1 side 002
vline gray

Rapid and Reliable Single Cell Dissociation

The TIGR Tissue Grinder and Dissociator is a novel semi-automated tissue dissociation system. Gentle mechanical dissociation yields high-quality single cells in a fast and reproducible manner to facilitate single cell analysis workflows.
vline gray 1

Applications

tissue grinder

The TIGR Tissue Grinder and Dissociator is the perfect tool for a wide range of applications, including, but not limited to:

vline gray

How it Works

The TIGR Tissue Grinder and Dissociator system is comprised of the benchtop instrument and a sterile, disposable 50mL grinding tube with an integrated core unit and cell strainer.

tigr 1
tigr 2
tigr tissue grinder rotor and stator teeth

The core grinding unit has two parts – the rotor and the stator inserts that interlock with rows of counter-rotating grinding teeth. The stator insert remains static while the rotator insert can rotate clockwise for  a shearing process or counterclockwise for a grinding/friction (milling) force. The rotation of the grinding teeth draws the tissue sample into the fin-shaped free space, applying adequate amounts of shearing and milling force to gently extract viable single cells from the tissue.

tigr tissue grinder workflow overview

The workflow is simple and standardized. In a sterile environment, the tissue sample is placed between the rotator and stator with a small volume of suspension buffer. The cell strainer and conical tube are placed over the grinding core, creating a sterile compartment for the dissociation to occur. The grinding tube is then placed in one of the four available slots in the TIGR system in a lid-down orientation so that the rotor connects to the slot’s rotation gear.

The easy-to-use TIGR software executes the grinding protocol parameters set for the experiment and tissue type. By inverting the griding tube with the lid up, the cell suspension flows through the cell strainer to filter the dissociated single cells from remaining tissue without the need for further processing. Centrifugation and resuspension of the cell pellet provides a single cell suspension ready for downstream workflows.