Who is using CellRaft Technology?
A Few Ways CellRaft® Technology is Being Used in Customer Labs
Scientific Poster: Accelerating Generation of Single Cell Clones
Poster: Leveraging a CRISPR-based LAPSE Workflow to Guide Drug Targeting Strategies
Case Study: Identification of a Rare Double Positive Clone
Journal Article: Detection of unintended on-target effects in CRISPR genome editing by DNA donors carrying diagnostic substitutions
Journal Article: A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A
Journal Article: Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations
Journal Article: PDS5A and PDS5B differentially affect gene expression without altering cohesin localization across the genome
Journal Article: RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells
Customer's Quotes
Rating 5.0 / review on SelectScience: "I can recommend it to anyone who wants to pick single cells or clone... It is user-friendly."
Reviewer: Moonsook Lee. Application Area: Whole genome amplification from single cells. Review Date: 13 Dec 2022 | CellRaft AIR SystemAn all-in-one platform for imaging, identifying, and isolating viable single cells, colonies, or organoids using CellRaft Arrays and software-guided selection with CellRaft Cytometry..
Rating Extremely Satisfied (5 Stars): “The system is great for difficult-to-clone cell lines and has powerful imaging technology to help with our cell engineering assays.”
Jillian Pattison, Senior Scientific Researcher.
“We recommend the CellRaft Technology for our colleagues struggling with single cell isolation. Even with help from FACS, although single cell clones could be obtained, from our experiences, CellRaft not only speeds up this process by providing many more clones but more importantly, the unique monitoring process provided by CellRaft supplies additional information for every single clone with a higher quality of clones.”
Pengda Liu, Ph.D., Associate Professor of Biochemistry and Biophysics.
“We can now easily identify thousands of single organoids with various morphologies (size, shape, complexity) and isolate hundreds in less than an hour. We also use the CellRaft AIR System for cloning of transgenic or CRISPR-edited human intestinal stem cells. Prior to the air system, the efficiency of isolating clonal lines was very inefficient because of the low throughput. Now we can isolate hundreds or thousands of clonal organoids in an unprecedented short amount of time compared to before.”
Scott Magness, Ph.D., Associate Professor, University of North Carolina – Chapel Hill, NC State University Joint Departments of Biomedical Engineering, UNC Departments of Medicine, Cell Biology, & Physiology Associate Professor of Biochemistry and Biophysics.
“The generation of these double positive clonal lines saved my lab of a lot of time and effort, allowing the rapid generation of multiple highly stable clones that had persistent expression for in vitro and in vivo assays. This proved superior to our flow-based methodologies, which were never as transgene positive or stable (in terms of expression) over multiple passages.”
Zachary Hartman, Ph.D., Director, Center for Applied Therapeutics. Associate Professor, Departments of Surgery, Pathology, and Immunology, Tumor Immunology and Immunotherapeutics Laboratory.
"At Colossal Biosciences, we only work with non-model organisms and primary cells and cell lines that haven’t been studied before. These cells often fail to grow out after monoclonal isolation using flow sorting. In the few months since adopting the CellRaft, we have isolated thousands of validated monoclonals from various species with consistent outgrowth efficiencies."
Dr. Sven Bocklandt, Species Director at Colossal where he is leading a team of genome engineers and cell biologists focused on bringing back extinct animal species.
“Our work routinely involves knocking in two or more unrelated fluorescent proteins into a single cell line by lentiviral methods. A significant challenge has been the isolation of strongly expressing all-positive clones for use as reporters. A more facile approach to knocking in and isolating such clones would greatly accelerate our work over engineering alternative construct designs. Cell Microsystems was able to isolate clones of THP-1 monocytes that are highly fluorescent for our assays. I would recommend this technology as a viable alternative to FACS, especially when cell populations are limiting.”
Gregory Poon, Ph.D., B.Sc.Phm., Professor of Chemistry, Georgia State University
“We employed the CellRaft Technology to help isolate clones derived from verified single cells in a cell line that was very difficult to clone by regular limiting dilution. It worked beautifully, producing clones with sequencing-verified distinct genotypes quite easily. This technology is definitely a big advance for anyone trying to isolate clones from single cells!!”
W. Brian Dalton, M.D., Ph.D., Asst. Professor of Oncology, Johns Hopkins Sidney Kimmel Comprehensive Cancer Center.
“As glioblastoma(GBM) cells need signals from other cells during culturing, we failed to obtain (a) single clone by isolating (a) single GBM cell into each well. I would like to recommend Cell Raft Technology if anyone has a problem in isolating single clones, especially those difficult culturing types… We got 26 single clones and validated them by DNA FISH. We got both ecDNA positive and negative clones (that) we need.”
Shu Zhang, Postdoctoral Researcher, Stanford University
"Several adherent lines tested in the lab do not survive sorting at high pressure and their outgrowth is poor when sorted singly in plates. Manually limiting dilution was previously the only process for obtaining clones; however, we can now utilize the CellRaft AIR® system for adherent lines as well."
Flow Cytometry and Cell Engineering Core, Kite Pharma, a Gilead Company.
“Because Hep3B cells are unable to undergo limiting dilution without differentiating, we had not attempted single cell expansions. When Hep3B cells differentiate, they lose the ability to respond to hypoxia…The CellRaft Technology allowed (the) expansion of a high percentage of colonies derived from a single Hep3B cell that faithfully retained the ability to respond to hypoxia, a critical measure of success for this procedure. Given their ability to generate clonal Hep3B population, I definitely recommend CellRaft Technology for use with cell types that are otherwise refractory to single cell cloning.”
Joseph Garcia, Professor of Medicine, Columbia University Medical Center/James J. Peters VA Medical Center