Who is using Cell Microsystems Technology?
A Few Ways Cell Microsystems' Products Are Being Used in Customer Labs
Scientific Poster: Accelerating Generation of Single Cell Clones
Poster: Leveraging a CRISPR-based LAPSE Workflow to Guide Drug Targeting Strategies
Case Study: Identification of a Rare Double Positive Clone
Journal Article: Detection of unintended on-target effects in CRISPR genome editing by DNA donors carrying diagnostic substitutions
Journal Article: A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A
Journal Article: Pooled image-base screening of mitochondria with microraft isolation distinguishes pathogenic mitofusin 2 mutations
Journal Article: PDS5A and PDS5B differentially affect gene expression without altering cohesin localization across the genome
Journal Article: RNA binding protein SYNCRIP maintains proteostasis and self-renewal of hematopoietic stem and progenitor cells
Journal Article: A Microfluidic Model of Microvascular Inflammation: Characterization and Testing of Endothelial-Targeted Therapeutics
Journal Article: Plasma components to protect the endothelial barrier after shock: A role for sphingosine 1-phosphate
Journal Article: Adhesion of Candida parapsilosis to Extracellular Matrix Proteins under Fluid Shear
Journal Article: New Device for High-Throughput Viability Screening of Flow Biofilms
Journal Article: A microtiter peg lid with ziggurat geometry for medium-throughput antibiotic testing and in situ imaging of biofilms
Journal Article: The protective role of estrogen on endothelial and glycocalyx barriers after shock conditions: A microfluidic study
Journal Article: Antibodies to PhnD Inhibit Staphylococcal Biofilms
Journal Article: Preliminary Characterization of NP339, a Novel Polyarginine Peptide with Broad Antifungal Activity
Journal Article: Synergistic Effect of Histamine and TNF-α on Monocyte Adhesion to Vascular Endothelial Cells
Journal Article: Well plate-based perfusion culture device for tissue and tumor microenvironment replication
Journal Article: Assessment and Optimizations of Candida albicansIn Vitro BiofilmA structured microbial community attached to a surface, typically more tolerant to antibiotics/biocides than planktonic cells. Assays
Journal Article: Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel
Journal Article: Neutrophil accumulation and NET release contribute to thrombosis in HIT
Journal Article: Platelet-Inspired Intravenous Nanomedicine for Injury-Targeted Direct Delivery of Thrombin to Augment Hemostasis in Coagulopathies
Journal Article: Magnesium Augments Platelet Activation and Coagulation in Citrated Blood Product
Journal Article: Interleukin-8 mediates neutrophil-endothelial interactions in pig-to-human xenogeneic models
Journal Article: Virstatin inhibits biofilm formation and motility of Acinetobacter baumannii
Journal Article: Examination of Staphylococcus epidermidis Biofilms Using Flow-Cell Technology
Journal Article: Survival, Biofilm Formation, and Growth Potential of Environmental and Enteric Escherichia coli Strains in Drinking Water Microcosms
Journal Article: Colistin-Resistant Acinetobacter baumannii Clinical Strains with Deficient Biofilm Formation
Journal Article: Evaluation of amygdalin-loaded alginate-chitosan nanoparticles as biocompatible drug delivery carriers for anticancerous efficacy
Journal Article: New Adapted In Vitro Technology to Evaluate Biofilm Formation and Antibiotic Activity Using Live Imaging under Flow Conditions
Journal Article: A high-throughput microfluidic dental plaque biofilm system to visualize and quantify the effect of antimicrobials
Journal Article: Laminar shear stressThe tangential force per unit area from flowing fluid; a key parameter in vascular biology, platelet function, leukocyte rolling, and biofilm adhesion studies. modulates endothelial luminal surface stiffness in a tissue-specific manner
Customer's Quotes
”...a recent demonstration of this technology in the lab has shown that, compared to current methods, the CellRaft AIR system increases the number of clones obtained by 5-fold and reduces the time needed to obtain those clones by 50% ...increasing the probability of obtaining a clone of interest while reducing waiting time.”
Rating 5.0 / review on SelectScience: "I can recommend it to anyone who wants to pick single cells or clone... It is user-friendly."
Rating Extremely Satisfied (5 Stars): “The system is great for difficult-to-clone cell lines and has powerful imaging technology to help with our cell engineering assays.”
“We recommend the CellRaft Technology for our colleagues struggling with single cell isolation. Even with help from FACS, although single cell clones could be obtained, from our experiences, CellRaft not only speeds up this process by providing many more clones but more importantly, the unique monitoring process provided by CellRaft supplies additional information for every single clone with a higher quality of clones.”
“We can now easily identify thousands of single organoids with various morphologies (size, shape, complexity) and isolate hundreds in less than an hour. We also use the CellRaft AIR System for cloning of transgenic or CRISPR-edited human intestinal stem cells. Prior to the air system, the efficiency of isolating clonal lines was very inefficient because of the low throughput. Now we can isolate hundreds or thousands of clonal organoids in an unprecedented short amount of time compared to before.”
“The generation of these double positive clonal lines saved my lab of a lot of time and effort, allowing the rapid generation of multiple highly stable clones that had persistent expression for in vitro and in vivo assays. This proved superior to our flow-based methodologies, which were never as transgene positive or stable (in terms of expression) over multiple passages.”
"At Colossal Biosciences, we only work with non-model organisms and primary cells and cell lines that haven’t been studied before. These cells often fail to grow out after monoclonal isolation using flow sorting. In the few months since adopting the CellRaft, we have isolated thousands of validated monoclonals from various species with consistent outgrowth efficiencies."
“Our work routinely involves knocking in two or more unrelated fluorescent proteins into a single cell line by lentiviral methods. A significant challenge has been the isolation of strongly expressing all-positive clones for use as reporters. A more facile approach to knocking in and isolating such clones would greatly accelerate our work over engineering alternative construct designs. Cell Microsystems was able to isolate clones of THP-1 monocytes that are highly fluorescent for our assays. I would recommend this technology as a viable alternative to FACS, especially when cell populations are limiting.”
“We employed the CellRaft Technology to help isolate clones derived from verified single cells in a cell line that was very difficult to clone by regular limiting dilution. It worked beautifully, producing clones with sequencing-verified distinct genotypes quite easily. This technology is definitely a big advance for anyone trying to isolate clones from single cells!!”
“As glioblastoma(GBM) cells need signals from other cells during culturing, we failed to obtain (a) single clone by isolating (a) single GBM cell into each well. I would like to recommend Cell Raft Technology if anyone has a problem in isolating single clones, especially those difficult culturing types… We got 26 single clones and validated them by DNA FISH. We got both ecDNA positive and negative clones (that) we need.”
"Several adherent lines tested in the lab do not survive sorting at high pressure and their outgrowth is poor when sorted singly in plates. Manually limiting dilution was previously the only process for obtaining clones; however, we can now utilize the CellRaft AIR® system for adherent lines as well."
“Because Hep3B cells are unable to undergo limiting dilution without differentiating, we had not attempted single cell expansions. When Hep3B cells differentiate, they lose the ability to respond to hypoxia…The CellRaft Technology allowed (the) expansion of a high percentage of colonies derived from a single Hep3B cell that faithfully retained the ability to respond to hypoxia, a critical measure of success for this procedure. Given their ability to generate clonal Hep3B population, I definitely recommend CellRaft Technology for use with cell types that are otherwise refractory to single cell cloning.”
"Thanks to the feedback from other users and the personal demo I received, I was quite confident about the performance of this equipment. Also, I quickly realized how efficient and reliable was the customer service. This is a great asset for the company."
"The great thing about BioFlux is that we can very precisely control the shear at which we drive fluid from the inlet to the outlet. That’s a godsend for us."
