CellRaft AIR® System

Image, Track, Analyze and Automate

The CellRaft Air® System is an integrated platform that uses proprietary technology to maintain cells in an unperturbed state, leading to improved viability of single cells, highly proliferative colonies, and superior clonal outgrowth, that provides a dramatic increase in the number of clones available for downstream applications. With the system you can:

Applications

On-Array Assays

Ideal Cell Types

P3.1 SeedCells
Seed Cells on CellRaft Array
Seeding cells on the CellRaft array is straightforward and comparable to seeding cells on any standard tissue culture dish. The dilute cell suspension is dispensed into the central reservoir, and cells settle into the microwells by gravity. The CellRaft Arrays are designed to yield a high number of single cells with the added benefit of shared media in a flask-like environment to increase viability and monoclonal outgrowth.
P3.1 SeedCells
P3.1 Image
Image Using CellRaft AIR System
Brightfield and fluorescence scanning provides single-cell resolution imaging in as little as seven minutes. CellRafts can be scanned over the days or weeks needed to identify samples of interest without monopolizing the instrument with a single experiment.
P3.1 Image
P3.1 Identify 2
Identify Cells of Interest in Software
CellRaft Cytometry™ allows for image-based verification of single cells to ensure monoclonality and analysis of a variety of parameters over time, ranging from morphology to gene expression. Users can easily define the characteristics of the target cells or colonies and map them for software-guided CellRaft selection for downstream isolation.
P3.1 Identify 2
P3.1 Automated Isolation
Automated Isolation to 96-well Plate
After identification, the CellRafts are gently dislodged from the CellRaft Array by a release needle and retrieved from the array by a magnetic wand to transfer the CellRafts into 96-well plates for downstream analysis. The fully automated CellRaft AIR System enables gentle, dissociation free isolation of fully validated, intact clones for propagation without dissociation or fluidics.
P3.1 Automated Isolation
Confluent colonies
(>50% confluent 10 days post-seeding)

iPSCs grown on a CellRaft Array. The CellRaft is isolated on Day 6 into a 96-well plate where the iPSCs continue to grow off of the CellRaft.

The flask-like culture conditions of the CellRaft Array combined with the automated and gentle isolation of identified cells, colonies, or organoids of interest results in a higher outgrowth percentage post-isolation than can be accomplished with limiting dilution or traditional cell sorters and dispensers.
array outgrowth black arrow 1
iPSCs grown on a CellRaft Array. The CellRaft is isolated on Day 6 into a 96-well plate where the iPSCs continue to grow off of the CellRaft.
Confluent Colonies
(>50% confluent 10 days post-seeding)
0%
Limiting Dilution
CellRaft Technology

No more limiting dilution. Grow more colonies. Save time and money.

CellRaft Technology offers an affordable, automated solution to an otherwise labor-intensive workflow while maintaining cell health, providing time-course imaging for clonal verification, and delivering automated isolation of cells, colonies or organoids.

Development of Monoclonal Cell Lines - Available Technologies and Overcoming Challenges

Have you ever wondered what impacts the viability and survival rate of your clones after a single cell cloning protocol? This white paper provides an overview of current methods for creating monoclonal lines from single cells and discusses the challenges and tips on how to overcome them.