CERO 3D Incubator & Bioreactor
Revolutionize Your 3D Cell Culture
Making the Impossible Possible
The CERO 3D Incubator & Bioreactor offers a unique 3D cell culture technology to boost your organoid, spheroid, stem cell, and tissue research. Low-shear, bi-directional rotation minimizes cell stress and damage, promoting long-term culture, survival, and differentiation without compromising cell health.
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Scalable and Efficient
Cultivate thousands of organoids per tube using minimal media. -
Reproducible
Consistent morphology and differentiation across replicates and cell lines. -
User-Friendly
Tubes resemble 50 mL conical for easy media changes and sampling. -
Environmental Control
Precise control of CO2, temperature, and pH ensures optimal conditions. -
Cost-efficient
Less media and no embedding substrates needed.
Save time. Enhance quality.
Pave new paths.
Meet CERO 3D
CERO Key Features
Beating of mature cardiac organoids in the CERO 3D.
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Innovative Design
Our patented bioreactor chambers are designed to facilitate the natural growth patterns of cells, allowing for nutrient flow and waste removal just as they would occur within a living organism. -
Versatility at Its Core
Whether you’re conducting research in oncology, stem cells, or toxicology, CERO adapts to your needs. Its compatibility with numerous cell types and matrices makes it a universal tool for every laboratory. -
Seamless Integration
CERO’s compact footprint and plug-and-play setup ensure that incorporating it into your lab is as straightforward as its operation.
Spheroid Formation of Edited iPSCs
Trusted by Hundreds of Labs Worldwide
Generation of HepaRG Spheroids in the CERO
Standardized generation of human iPSC‑derived hematopoietic organoids and macrophages
3D Cell Culture CEROplates
- 96-well, U-bottom shaped, ready-to-use plates
- Support uniform spheroid and organoid formation
- Allow long-term 3D culture over months
- Anti-adhesive surface inhibits cellular attachment
- Compatible with existing imagers, liquid handling, and automated stations
- Improves data consistency
- Suitable for high-throughput applications
The ultra-low attachment CEROplate simplifies the process for growing 3D aggregates. It features clear wells with a U-bottom to make sample monitoring simple. The unique well geometry of the microplate aids in the formation of an unattached, round-shaped, single spheroid or organoid in the center of each well.
This allows you to assay and analyze your 3D aggregates in the same plate without transfer. The CEROplates are compatible with existing readers, imaging systems, liquid handling, and automated workstations.
The CellRaft AIR® Is the Perfect Complement to the CERO 3D
Transform Your Organoid Workflow: The CellRaft AIR® + CERO 3D
- Maintain a complete record of organoid growth over multiple time points
- Perform on-array phenotypic assessment
- Fully automate isolation of individual viable, intact organoids of interest
- Generate customized 96-well plates for organoid-based assays such as clonal propagation, drug or toxicity screening, or target discovery
CERO 3D FAQs
How can I grow consistent, uniform 3D spheroids or organoids?
Achieving uniform 3D structures requires tight control of environmental conditions and low-shear culture. The CERO 3D incubator and bioreactor provides a controlled, low-stress suspension environment that produces highly homogeneous aggregates without microcarriers or scaffolds.
What is the best way to maintain long-term 3D cell cultures with high viability?
Long-term viability depends on stable pH, CO₂, temperature, and gentle mixing. The CERO 3D maintains a fully regulated culture environment, enabling 3D spheroids, organoids, and stem-cell aggregates to remain healthy for weeks to months.
How do researchers scale up 3D cultures for downstream assays or manufacturing?
Scaling up requires a system that supports large culture volumes without compromising uniformity. The CERO 3D allows expansion of 3D models in up to ~50 mL culture tubes, enabling reliable biomass generation for screening, toxicity testing, and disease modeling.
How can I reduce shear stress during 3D suspension culture?
Traditional shakers and spinner flasks can damage delicate aggregates. The CERO 3D uses a low-shear rotation system that protects developing spheroids and organoids while ensuring uniform nutrient and oxygen exposure.
What’s the best solution for generating reproducible 3D models across multiple experiments?
Reproducibility requires consistent environmental conditions and standardized vessel geometry. The CERO 3D uses identical disposable CEROtubes and closed-loop environmental control to achieve experiment-to-experiment consistency.
How do I minimize hands-on time when growing organoids or spheroids?
Automated mixing and environmental monitoring dramatically reduce manual intervention. The CERO 3D supports “set-and-walk-away” culture with only brief daily checks, helping labs save hours of effort per week.
How can I improve the yield of iPSC-derived organoids or 3D tissues?
Higher yields come from stable culture conditions and consistent mixing. The CERO 3D produces dense, viable 3D biomass with minimal cell death, supporting applications such as drug discovery, disease modeling, and regenerative research.
