Stem Cells
Are iPSCs a Bottleneck in Your Lab?
Generate 100s of healthy monoclonal iPSCs or hESCs
- Increase clonal iPSC generation by 25X compared to limiting dilution
- Track and trace iPSCs from single cell to clone
- Expand, characterize, edit, and reprogram
- Confirm pluripotency on-array prior to clonal expansion
- Reduce time, consumables, materials, and labor required for iPSC clonal development
- Enable 2D and 3D applications including cell line development, reprogramming, and differentiation
- Fully automate the gentle isolation of viable, validated iPSC cell lines
Increase in iPSC Monoclonal Colony Generation and Outgrowth
The CellRaft AIR SystemAn all-in-one platform for imaging, identifying, and isolating viable single cells, colonies, or organoids using CellRaft Arrays and software-guided selection with CellRaft Cytometry. is able to screen more than 60,000 individual iPSCs per CellRaft Array which is 500X more cells than a traditional 96-well plate with limiting dilutionA statistical cloning approach where cells are diluted to low density to “hopefully” seed one cell per well; commonly used but can be low-efficiency for difficult-to-clone lines.. This increases your likelihood of success in these workflows.
Track and Trace from Single Cell to Clone
Follow the maturation of a clone as it grows from a single cell.
Enable All iPSC Workflows Using CellRaft Technology
- Expand the clonal population on the array
- Characterize for phenotyping or pluripotency
- Differentiate into 3D tissues or organoids
- Reprogram differentiated fibroblasts into pluripotent stem cells
Application Notes: No more manually scraping cells: An automated efficient solution to iPSC reprogramming
Reduce Time, Consumables, Materials, and Labor
The labor, cost, and reagent burden associated with iPSC maintenance and workflows can be incredibly high and often prohibitive for many laboratories.
We compared clonal iPSC development using CellRaft Technology to traditional limiting dilution. Using the CellRaft Array, we were able to generate over 200 single cell-derived iPSC clones on a single array, compared to 10 clones in a single 96-well plate with limiting dilution. This workflow required 1000X less iPSC coating and 2000X less media per cell screened.
Grow and Isolate iPSC-derived Organoids
CellRaft Technology can be used to grow and maintain hundreds of individual organoids. You can serially image the same organoidA 3D, self-organizing cell structure, derived from pluripotent or adult stem cells that mimic aspects of organ function and architecture. over time and phenotypically characterize organoids to identify the ones of interest.
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