Antimicrobial Screening

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The BioFlux system is a microfluidic platform designed to run automated shear flow protocols microbiology experiments. A 48-well BioFlux plate can run up to 24 experiments simultaneously. To increase throughput even further, the BioFlux Quattro system operates up to 96 simultaneous experiments, making it ideal for antimicrobial screening assays on biofilms. 

In the paper below, various compounds were tested to determine their ability to prevent or eradicate biofilms.

s mutans
(A) S. mutans biofilms were cultured in a BioFlux system for 18 to 24 hours, inoculated with different antibacterial agents, and stained (live cells stained green and dead cells stained red). (B) These results were graphed, showing statistically significant differences between the control and the antimicrobial-treated groups (Ding et al., 2014).
antimicrobial screening s mutans
S. mutans was grown on a BioFlux plate, then treated with pleurocidin, and stained (green being the live cells and red being dead). Bacteria on the outside of the biofilm was killed by the antimicrobial agent, whereas the bacteria closer to the inside of the biofilm was protected by the surroundings (Tao et al., 2011).

Key BioFlux Advantages For Antimicrobial Screening Studies

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dioscin under flow biofilms table
Article Highlight

In Silico Screening and In Vitro Assessment of Natural Products with Anti-Virulence Activity against Helicobacter pylori

Helicobacter pylori is one of the most frequently encountered human pathogens. Infection with H. pylori can lead to the development of various gastric diseases, such as peptic ulcers and gastric cancers. 

In this article, Spiegel et al. virtually screened 791 natural substances for anti-biofilm activity. To find the most effective antimicrobial substance, the group leveraged the high throughput capacity of the BioFlux 1000Z to test the 10 top candidates under physiological flow and no flow environments.

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Featured Poster

Examination of biofilms grown in microfluidic flow cell arrays for compound screening, host-pathogen interactions, and microbial adhesion

Higher-throughput biofilm assays often employ well plates for compound, mutant and conditional screening. Despite their throughput and convenience, well-plate assays have difficulty producing
higher content biofilm data, such as morphology, compound penetration, analysis of growth under flow, and analysis of mixed species interactions.

We present the BioFlux microfluidic flow cell array, which incorporates high content flow-based biofilm experiments integrated into a standard well-plate format.

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