Organoids

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Transforming How Organoids are Grown and Isolated

In typical organoid cultures, the structures overlap, requiring multifocal imaging with expensive equipment. They grow at different sizes and rates, leading to heterogeneity in viability and phenotype, resulting in confusing and uninterpretable data. Organoids grown in bulk culture are stuck in viscous extracellular matrix and are difficult to retrieve intact.

The CellRaft AIR® System with 3D capabilities offers a solution with a fully automated imaging and isolation workflow for organoid culture.

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Image

Capture  images in brightfield and fluorescence throughout the full height of the organoid with z-stack imaging.

The images showing detailed phenotypic characterization are cataloged and easily exported for the desired use.

imagesoftware
Our user-friendly proprietary software enables a one-touch full-array image scan
Brightfield
Brightfield Imaging of Mouse Hepatic Organoids
z stack
EpCAM & Hoechst Imaging of Mouse Hepatic Organoids
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Identify

Identify CellRafts containing 3D structures of interest using CellRaft Cytometryâ„¢ to detect organoids. Visually verify single cell clonality, morphological data, and growth rate, critical data points for workflows requiring track and traceability to confirm clonality. Identified organoids can be further evaluated and selected for phenotypic characteristics, such as organoid diameter, circularity, and fluorescence intensity.

Identify Phenotypic
Perform phenotypic characterization with Raft Cytometry.
Identify Software
Identify and verify CellRafts containing 3D structures of interest and create sets of sub-populations for isolation
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Isolate

The Air System with 3D capabilities enables automated isolation of live, intact organoids without fixation, dissociation, or damage to the 3D structure. Organoids remain viable through isolation and continue to grow off-array.
Pre isolation
Clonally-derived Organoid on a CellRaft®
post isolation
Organoid is isolated on the CellRaft
Day1
Day 1 Organoid Growth
day5
Day 5 Organoid Growth
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