CRISPR

Improve viability, clonality, & efficiency

Identify target cells on Day 0.  Track single cells and their phenotypes through the cloning and sorting process. See the quality of your cells before collection and downstream analysis.  All on one fully automated platform using one consumable.

Three to four days of hands-on time for dozens of cell lines.

 

Increase cell viability several fold when compared to FACS.

How It Works

The process of cellular cloning and sorting is a major bottleneck in the CRISPR/Cas workflow.  The imaging capabilities of the CellRaft AIR System reduces this bottleneck by providing the user with Day 0 scans to visually connect CRISPR edits to desired phenotype expression at the single cell level.  The user can identify clones of interest and confirm that clonal colony propagation began from a single cell. 

After cells are introduced to the Cas enzyme and guide RNAs, they are immediately plated on a CytoSort Array, eliminating the need for trypsin, scraping, high pressure fluidics, and limiting dilution.  The array is scanned and imaged, allowing the user to easily identify which cells to target and track through the initial colony growth phase.

Target cells grow unperturbed in optimized conditions on the CytoSort Array, increasing cell viability, while still allowing growth of target clonal colonies to be monitored.  The CytoSort Quad and HexaQuad Arrays allow parallel gene editing and cloning processes, making it possible to generate four to 24 cell lines on a single consumable.

When colonies have reached the desired size and density, the colony CellRaft is released, collected, and transferred to an expansion culture plate for downstream analysis.

Click here for CRISPR tips and a more detailed methodology