IonFlux Consumables
IonFlux Plates
Unique in-plate liquid handling, patching and recording
IonFlux uses a unique plate design that allows for all fluid activity to be conducted within the recording plate proper. Using flexible and user controlled pneumatic system, IonFlux provides high speed liquid exchange, continuous solution flow, and drug delivery without the use of an external liquid handler. The in-plate movement of solutions cancels typical ion channel assays problems such as stacking related drug cross contamination. The continuous flow system alleviates assays with slow acting or sticky compounds.
Design
IonFlux Plates are microfluidic devices used exclusively in the IonFlux System for automated patch clamp assays. They are integrated into SBS-standard well plate formats for convenience and compatibility with automated liquid handling instrumentation. Each well on the plate has a dedicated function as either a compound well, cell trapping well, cell inlet or cell outlet well.
Plates are divided into experimental patterns. Each pattern contains specified wells:
- Two Independent trapping wells: These wells would contain intracellular solutions
- Cell inlet well: This well would contain the prepared cells in extracellular solution
- Cell outlet well: Acts as a waste well for the pattern
- Eight independent compound wells: These wells would contain any combination of different compound solutions or dose response concentration gradient of the same compound. IonFlux software provides full control over these compound wells release and timing.
Format
IonFlux plates are 96-well plate format for use with lonFlux Mercury 16.
Recording
- Single Cells: Using whole cell patch clamp technique and achieving Giga-Ω seal recordings, lonFlux single cell plates are perfect for specialized assays requiring complete voltage/current control of an independent cell, mimicking manual patch clamp conditions while providing automated systems flexibility.
- Ensemble cells: Just like single cell plates, ensemble plates also use whole cell patch clamp techniques but now with 20 cells in parallel, setting a standard for consistency and throughput.
All IonFlux instruments can use either single-cell or ensemble plates with no hardware modifications. To change modes, simply use a different plate.
Plate Types
IonFlux plates are shipped in boxes of 8 pieces.
IonFlux Plate 16 (ENSEMBLE)
PART# 910-0054