Trypsin-free isolation

Definition

Cellular recovery method that avoids enzymatic detachment, which can reduce stress and phenotypic perturbation during selection/removal.

Why it matters

For scientific teams:

Preserves sensitive surface proteins.
Improves post-isolation viability.
Reduces perturbation before downstream assays.

For decision-makers:

Improves clone recovery rates.
Reduces variability introduced by processing.
Supports higher-quality cell products.

FAQ

Q: Why avoid trypsin during isolation?

A: Trypsin can cleave surface proteins and stress cells.

Q: When is trypsin-free isolation most important?

A: When viability and surface marker integrity are critical.