Enzyme-free dissociation
Definition
Disruption of cells or tissues without enzymatic digestion (useful when enzymes may alter epitopes or expression profiles). TIGR highlights enzyme-free, fast processing.
Why it matters
For scientific teams:
- Maintains epitope integrity for downstream labeling or sorting.
- Reduces stress-induced transcriptional changes.
- Improves comparability between samples.
For decision-makers:
- Reduces variability linked to enzyme performance.
- Improves reliability of marker-dependent assays.
- Supports higher-quality single-cell data generation.
FAQ
Q: Why avoid proteolytic enzymes?
A: They can cleave surface proteins and perturb cell physiology.
Q: Is enzyme-free dissociation always sufficient?
A: Some dense tissues may still require enzymatic assistance.