Single-cell clone generation remains a major bottleneck in cell engineering. Current practices vary from manual limiting dilutionA statistical cloning approach where cells are diluted to low density to “hopefully” seed one cell per well; commonly used but can be low-efficiency for difficult-to-clone lines., to using a variety of high-speed cell sorters, or a combination of both.
Join us for a series of case studies highlighting how scientists struggling with generating clones were able to overcome common bottlenecks in their research. Labor burden, poor cell viability, low efficiency, and lack of clonality are just some of the hurdles these investigators faced in developing the cell lines that are necessary for downstream discovery.
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