Cellular migration is an important biological process in both health and disease. Chemical messengers, known as chemokines, induce numerous steps of cell communication and responses that direct cells to tissues. To examine detailed cellular migratory processes in a physiologically relevant manner over time, it is necessary to observe cells in situ. Unlike typical Boyden chamber or transwell migration assays, a BioFlux system allows for a broad analysis of the entire chemotactic process along with environmental control, while requiring considerably less hands-on time.