This experiment utilized our 24-well plates, which have two flow channels that merge into a common flow cell. This creates two distinct environments with a diffusional boundary in the middle, allowing for unique chemotaxis and migration assays under shear stressThe tangential force per unit area from flowing fluid; a key parameter in vascular biology, platelet function, leukocyte rolling, and biofilm adhesion studies.. Conditions can be varied by fluid type, gas composition, pH, etc. The paper can be accessed here: https://www.pnas.org/doi/10.1073/pnas.1600760113.