In this Application Note, we explore CellRaft® Technology’s ability to maintain the benefits of bulk cell culture, while also providing single cell track and trace resolution.
Key Highlights:
1) The main failure mode for cell line development is the inability of an isolated single cell to generate a viable colony.
2) Subjecting cells to manipulations such as fluidics or transfection contributes to the poor efficiency of single cell cloning.
3) Isolating colonies rather than single cells leads to successful cell line development, even for the most challenging or fragile cell lines.
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