In this Application Note, we explore how CellRaft® Technology streamlines reprogramming by improving efficacy and efficiency compared to traditional methods.
Key Highlights:
1. The CellRaft AIR SystemAn all-in-one platform for imaging, identifying, and isolating viable single cells, colonies, or organoids using CellRaft Arrays and software-guided selection with CellRaft Cytometry. enables the automated isolation of hundreds of pluripotent colonies in half the time compared to manual scraping, pruning, or aspiration.
2. Reprogramming with the CellRaft ArrayConsumables containing thousands of microwells (containing CellRafts) for spatial segregation, imaging, and isolation of single cells, monoclonal colonies, or 3D cultures. generated thousands of individually segregated TRA-1-60 positive colonies.
3. Using the CellRaftA microscale polystyrene growth surface within an array used to spatially segregate cells/colonies while maintaining shared media access, enabling imaging over time and targeted isolation. Array, stable monoclonal iPSC cell lines can be derived and characterized up to four weeks sooner than the traditional method.
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