Platelet Aggregation & Adhesion
Hematology with BioFlux
Traditional platelet aggregation assays, such as light transmission and multiple-electrode aggregometry, rely on aggregation agonists in static conditions, thereby limiting their physiological relevance. In addition, these assays lack the ability to examine cell-cell interactions, platelet adhesion to various substrates, and modeling platelet function in various areas of the vasculature by assaying under multiple shear stresses.
BioFlux shear flow systems overcome these disadvantages by enabling the visualization and analysis of platelet aggregation and adhesion under physiological and pathophysiological shear stresses. High-throughput microplates, combined with versatile sample capabilities including whole blood can reduce assay time to 3 hours, making BioFlux the ideal system to investigate platelet function in disorders such as COVID-19 and Sickle cell disease. Â Â Â Â
One system, many assays
BioFlux hematology assays:
- Platelet rolling
- Platelet adhesion & adhesion inhibition
- Platelet aggregation
- Thrombus formation & reversibility
- Drug discovery & development
- Assessment under variable flow rates
- Analysis under pulsatile flow
Key BioFlux Advantages For Platelet Aggregation & Adhesion Studies
- Physiological assessment of adhesion and aggregation using primary endothelial cells, human peripheral blood mononuclear cells, and more
- Assay platelet adhesion and aggregation kinetics in about 3 hours
- Utilize time-lapse microscopy and post-experiment image analysis to determine rolling distance under flow
- Examine up to 24 different monolayers on one plate to determine IC50 or screen multiple compounds under identical conditions
- Calculate area of platelet adhesion by utilizing Montage Plus analysis capabilities
BioFlux platelet aggregation & adhesion assay general workflow
Hematology assays using BioFlux
Efficacy of platelet-inspired hemostatic nanoparticles on bleeding in von Willebrand disease murine models
FAQs:
Platelet Aggregation and Adhesion Using the BioFlux
How can I model platelet adhesion in vitro using microfluidics and shear flow?
The BioFlux is a versatile microfluidic platform optimized for modeling platelet adhesion and aggregation in vitro. It enables the controlled perfusion of whole blood or blood components under precisely regulated shear stressThe tangential force per unit area from flowing fluid; a key parameter in vascular biology, platelet function, leukocyte rolling, and biofilm adhesion studies. to simulate arterial and venous environments while supporting real-time observations of platelet interactions on microfluidic channels coated with relevant substrates (e.g., collagen, fibrinogen, Von Willebrand Factor) and endothelial cell layers.
What tools are available for visualizing platelet thrombus formation in real time?
The BioFlux System integrates seamlessly with live-cell imaging setups, including phase contrast and fluorescence microscopy. Researchers can visualize thrombus formation, platelet rolling, and aggregation in real time, capturing high-resolution data for quantitative analysis.
Can the BioFlux be used to study platelet function in disease models like thrombosis or atherosclerosis?
Yes, the BioFlux is widely used in translational research to study platelet behavior in disease models. Its ability to simulate vascular microenvironments makes it ideal for investigating platelet activation, aggregation, and adhesion in conditions such as thrombosis, atherosclerosis, and inflammation.
What are the advantages of using the BioFlux over traditional static platelet assays?
Unlike static assays, the BioFlux replicates physiological shearFlow conditions intended to resemble those in vivo (e.g., vasculature, catheters, mucosal surfaces). stress, which is critical for accurate platelet function studies that better reflect in vivo conditions.
How does BioFlux enable simultaneous testing of multiple platelet aggregation conditions?
The BioFlux is the only microfluidics-based platform that supports multi-condition testing through its unique plate formats. With plates containing between 3-24 microfluidic channels, researchers can run multiple experimental conditionssimultaneously undercontrolled shear flow—ideal for comparative studies, drug screening, biomarker validation, and mechanistic studies of platelet biology.
What substrates can be used in the BioFlux channels for platelet adhesion studies?
Common substrates include collagen, fibrinogen, von Willebrand factor, and endothelial cell monolayers. BioFlux channels can be easily coated to mimic vascular surfaces, allowing for targeted studies of platelet-surface interactions.
Is the BioFlux compatible with whole blood for platelet aggregation assays?
Yes, the BioFlux is compatible with whole blood, platelet-rich plasma, or other blood components. Its controlled perfusion makes it suitable for physiologically relevant aggregation assays.
