Pulsatile Flow Module – Use of variable shear to simulate vascular conditions – Technical Note
Archives: Application Notes
Restoring high-titer antibody production in a hybridoma cell line
Hybridomas are generated through the fusion of non-proliferative primary B cells with immortalized myeloma cells, followed by a labor- and resource-intensive single cell screening and cloning process to obtain a monoclonal hybridoma cell line.
Experimental variance of a BioFlux adhesion assay – Technical Note
The Stem Cell Encyclopedia
What We Have Learned About High-Efficiency Single Cell Cloning of Human Pluripotent Stem Cells Pluripotent stem cells (PSCs) hold the potential to transform medicine as cellular therapeutics and models of disease with their unique ability to differentiate into any somatic cell type. CellRaft® Technology provides an integrated platform for gene editing, differentiation, and single cell […]
Cell Line Development of Sf9 Cells Overcoming Challenges and Bottlenecks
The baculovirus expression vector system (BEVS) is a versatile method for producing recombinant proteins in insects. However, single cell cloning, ideal for stable production cell lines, is challenging with Sf9 cells, causing bottlenecks in BEVS workflows. These cells are sensitive to stressors like low seeding density and high sorting, leading to a reliance on polyclonal […]
No more manually scraping cells: An automated efficient solution to iPSC reprogramming
In this Application Note, we explore how CellRaft® Technology streamlines reprogramming by improving efficacy and efficiency compared to traditional methods. Key Highlights: 1. The CellRaft AIR System enables the automated isolation of hundreds of pluripotent colonies in half the time compared to manual scraping, pruning, or aspiration. 2. Reprogramming with the CellRaft Array generated thousands […]
Unlock the potential of developing cell lines from a menagerie of species
In this Application Note, we explore CellRaft® Technology’s ability to overcome the challenges of working with genetically and species-diverse cell lines. Questions this Application Note answers: 1. How do I successfully culture diverse cell types? 2. Is there a better alternative to limiting dilution for generating monoclonal cell lines? 3. What is the best way […]
Accelerate Cell Line Development by Isolating Monoclonal Colonies, Not Single Cells
In this Application Note, we explore CellRaft® Technology’s ability to maintain the benefits of bulk cell culture, while also providing single cell track and trace resolution. Key Highlights: 1) The main failure mode for cell line development is the inability of an isolated single cell to generate a viable colony. 2) Subjecting cells to manipulations such […]