Atherosclerosis Models
A Method to Culture Endothelial Cells in the Presence of Cyclic WaveformsÂ
Download this poster to learn how to develop physiologically relevant athero-prone and athero-protective endothelial cell models.
Poster highlights include:
- Using shear stressThe tangential force per unit area from flowing fluid; a key parameter in vascular biology, platelet function, leukocyte rolling, and biofilm adhesion studies. to simulate the effects of atherosclerosis on different areas of the vasculature
- The differences in the impact of continuous or pulsatile shear flowFluid movement that applies force to cells and biofilms; used to mimic physiological/pathological conditions. on endothelial cell organization
- How to improve the physiological relevance of cell culture modelsÂ
Atherosclerosis modeling with BioFlux
Atherosclerosis is one of the leading causes of death worldwide. The mechanisms of atherosclerotic plaque formation, growth, and treatment are difficult to study in vivo due to the large hurdles of time, expensive animal models, and difficulty imaging cellular events.
BioFlux shear flow systems clear these hurdles by leveraging microfluidics that enable investigators to mimic in vivo processes that occur in the development and progression of atherosclerosis, such as endothelial cell phenotype and functional changes, immune cell chemotaxis, adhesion, transmigration, platelet adhesion, and foam cell growth. Â
BioFlux plates enable the collection of high-resolution images and/or videos in real-time while conducting up to 24 assays simultaneously.
Key BioFlux Advantages For Atherosclerosis Studies
- Test for novel compounds to treat inflammation during the development of atherosclerosis
- Treat channels with atherosclerotic plaque from patients to enhance physiological relevance of assay
- Use low levels of shear stress to stimulate endothelial cell proliferation, coagulation, and apoptosis
- Utilize high levels of shear stress to encourage anticoagulation and endothelial cell survival
- Inhibit platelet and monocyte adhesion under shear flow to assess atherosclerosis prevention
BioFlux atherosclerosis workflow
Peer-reviewed atherosclerosis investigations using BioFlux
Diabetes is accompanied by secretion of pro-atherosclerotic exosomes from vascular smooth muscle cells
Rac1-mediated signaling plays a central role in secretion-dependent platelet aggregation in human blood stimulated by atherosclerotic plaque
Endothelial Junctional Adhesion Molecule-A Guides Monocytes Into Flow-Dependent Predilection Sites of Atherosclerosis
