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Publication: Acute hyperglycemia increases sepsis related glycocalyx degradation and endothelial cellular injury: A microfluidic study

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Human umbilical vein endothelial cell (HUVEC) monolayers were established in BioFlux microfluidic channels and activated with TNF. Endothelial glycocalyx (EG) integrity was indexed by shedding of the EG components as well as thickness. Endothelial cell (EC) injury/activation was indexed by soluble biomarkers. Intracellular reactive oxygen species (ROS) was by fluorescence. The investigators concluded that BioFlux may be a useful platform to study endothelial barrier function and stress conditions and allow preclinical assessment of potential therapies.

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