Leukocyte Transmigration Under Physiological Flow
Archives: Application Notes
High Throughput Cell Adhesion
Platelet Adhesion and Aggregation Assays
In the cardiovascular system, shear stress generated by the blood flow in the circulation impacts platelet receptor expression and function. Therefore, understanding the complex biological relationships that contribute to hemostasis and thrombosis requires accurate physiological analysis of platelet function under shear flow.
Key highlights of this application note include:
1) Adherence to multiple substrates in the same experiment
2) High-throughput pharmacological screening
3) Easily replicate in vivo platelet/vascular interactions
Cellular Migration and Invasion Assays
Cellular migration is an important biological process in both health and disease. Chemical messengers, known as chemokines, induce numerous steps of cell communication and responses that direct cells to tissues. To examine detailed cellular migratory processes in a physiologically relevant manner over time, it is necessary to observe cells in situ. Unlike typical Boyden chamber or transwell migration assays, a BioFlux system allows for a broad analysis of the entire chemotactic process along with environmental control, while requiring considerably less hands-on time.
Host-Pathogen Interactions
Biofilm Growth: Growth of biofilms using the BioFlux System
Making the Impossible Possible: Platform and Protocols to Develop Clonal iPSC Derived Organoids
In the last decade, several protocols and commercial kits have been launched to help differentiate iPSCs into multicellular, neuronal organoids that closely resemble human brain development, including region-specific cellular composition and functional physiology. However, the adoption of these organoid models is still limited to relatively low throughout applications, as the workflows are hampered by challenges in reproducibility and scalability, as well as being manually intensive. Here we report the use of the CellRaft® Technology, to develop and enable streamlined, reproducible organoid workflows that offer reliable imaging, software-guided selection, and automated isolation of single organoids for downstream applications.
Taking the Fear Out of iPS Cell Line Development
Despite significant strides in technology and increased access for researchers, iPSC culture remains a challenge, thereby limiting its widespread utility for therapeutic and research use. Common pain points include:
1) iPSC lines are sensitive and easily perturbed, requiring constant maintenance.
2) Poor culture conditions and cell line instability can lead to spontaneous differentiation and loss of pluripotency.
3) Generating monoclonal cell lines is incredibly challenging, with low efficiency and lack of proven clonality.
4) The labor, cost, and reagent burden associated with iPSC maintenance and workflows are incredibly high and often prohibitive for the end user.
Download this RaftNote to see how some of these challenges can be overcome.