Contact Us

Resources

Publication: An epilepsy-associated mutation in the nuclear import receptor KPNA7 reduces nuclear localization signal binding

View or download the Publication to read more
This study aimed to understand more about KPNA7 nuclear import receptor and its link to epilepsy. HeLa cells were transfected with ViaFect reagent before being seeded on a CellRaft Array. GFP-positive clonal CellRafts were identified and isolated using the AIR System. The cell lines generated using the CellRaft Array and AIR System were used for permeabilized cell import assays to show nuclear accumulation of GST-GFP-SV40 NLS. The data generated in this study supported a role for altered neuronal expression and activity of KPNA7 in a rare type of pediatric epilepsy.

Related Resources

Distinct and convergent effects of SF3B1 mutations in human breast cancer

In this study, CellRaft AIR was used to isolate, verify, and expand monoclonal cell lines carrying engineered SF3B1 mutations, forming the basis of an isogenic...

Learn more

A benchmark of the somatic mutation landscape using single-cell and single-molecule whole-genome sequencing

In this work, CellRaft arrays were used to generate clonally derived cell populations from single cells prior to sequencing, providing a ground-truth reference for benchmarking...

Learn more

Loss of cell cycle gatekeeping by CNOT3 impairs hematopoietic stem and progenitor cell division and repopulating activity

In this study, CellRaft AIR was used to isolate and track single hematopoietic stem and progenitor cell (HSPC) clones over time, enabling researchers to directly...

Learn more
See all Publications