Contact Us

Resources

Publication: Application-Oriented Bulk Cryopreservation of Human iPSCs in Cryo Bags Followed by Direct Inoculation in Scalable Suspension Bioreactors for Expansion and Neural Differentiation

View or download the Publication to read more
In this study the CERO 3D Incubator and Bioreactor was used for cultivation of different human iPSC lines and their neural differentiation. The article presents a study on bulk cryopreservation of the human iPSC lines UKKi011-A and BIONi010-C-41, which are stem cells with promising applications in regenerative medicine. The article discusses the method used for bulk cryopreservation, the results obtained after thawing and seeding the frozen cells in scalable suspension-based bioreactors for expansion and neural differentiation, and the conclusion that this technology could help avoid pre-cultivation and enable time- and cost-efficient therapeutic approaches with bulk cell numbers.

Related Resources

Centenarian SIRT6 variants elevate SIRT6 protein and enhance cellular senescence resistance

Researchers at Columbia University used the CellRaft AIR System to support the development of precisely engineered human embryonic stem cell (hESC) lines for a study...

Learn more

Distinct and convergent effects of SF3B1 mutations in human breast cancer

In this study, CellRaft AIR was used to isolate, verify, and expand monoclonal cell lines carrying engineered SF3B1 mutations, forming the basis of an isogenic...

Learn more

A benchmark of the somatic mutation landscape using single-cell and single-molecule whole-genome sequencing

In this work, CellRaft arrays were used to generate clonally derived cell populations from single cells prior to sequencing, providing a ground-truth reference for benchmarking...

Learn more
See all Publications