Unparalleled Single Cell Workflows

Generate more monoclonal colonies from single cells, faster with less effort

 

Stem Cells

A gentle way to handle sensitive cells

Improving viability, clonality, & efficiency on a single consumable

Fast, effective, and gentle to maintain native expression profiles

Organoids

Simple workflow for a novel and powerful technology 

Empowering the Generation and Automated Isolation of
Monoclonal Colonies from Single Cells

If your team is having challenges in generating enough viable monoclonal colonies from single cells and if limiting dilution, flow sorting, or fluidics instruments are consuming time, capital, and lab resources without yielding enough clones or organoids, then CellRaft® Technology may be the solution for you.

Over the past 10 years, we have assembled a team of biologists, engineers, and software developers to solve the problem of inefficient single cell cloning and low yields of monoclonal colonies. We have taken the approach of developing a method where single cell isolation and cloning achieve the highest level of success compared to anything on the market. We have tested more than 100 cell lines with CellRaft Technology to demonstrate the high outgrowth efficiencies using our platform.

With the unique CellRaft Technology you can:

  • Grow single cells without physically isolating cells, thus no perturbation to ensure the viability of single cells as they develop into clones.
  • Obtain 10 to 50X more viable monoclonal colonies with a high proliferative index (vitality).
  • Image, track, analyze, and automate isolation of colonies from single cells on one instrument (three-color and brightfield) with no minimum sample size requirement.
  • Identify the cells you want the instrument to automatically isolate based on various precise user-based attributes as a function of gene expression-morphology and time using the CellRaft Cytometry™ software.
  • Grow colonies from primary, adherent, or suspension cells, including iPSCs and immune cells
  • Grow iPSCs into 3D cell systems, including organoids.
  • Maintain a complete record of all clones to accelerate regulatory submissions.

Several adherent lines tested in the lab do not survive sorting at high pressure and their outgrowth is poor when sorted singly in plates. Manually limiting dilution was previously the only process for obtaining clones; however, we can now utilize the CellRaft AIR® system for adherent lines as well.

─Poster presented at CYTO 2022 by Kite Pharma Scientists in the Flow Cytometry and Cell Engineering Core

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Download Poster

Together with the CellRaft AIR System, CellRaft Arrays gently sort cells, resulting in cells that are highly viable with unperturbed phenotypes.

CellRaft® Array

Still Doing Limiting Dilution?

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