As drug discovery and disease modeling become increasingly sophisticated, 3D cell culture has emerged as a powerful alternative to traditional 2D assays and animal models. In a recent webinar Dr. Allysa Stern showcased how researchers are using the CERO 3D Incubator and Bioreactor to streamline development of organoid models that are scalable, reproducible, and biologically […]
The World Health Organization stated that as of 2020, nearly 8 million women alive were diagnosed with breast cancer in the last 5 years, making it the world’s most prevalent cancer (Retrieved from WHO). Globally, 1 in 8 women will be affected at some point during their lives with breast cancer and must undergo surgery […]
Generating edited monoclonal cell lines has become the first, and often the most important, step in therapeutic discovery pipelines. From editing to clonal expansion, cell line development is a laborious and expensive endeavor that can often be a rate-limiting step in bringing new therapeutics to market. One of the most common ways of generating clonal […]
You never forget the first time you thaw a vial of induced pluripotent stem cells. Standing in the lab, holding a precious vial of cells, reading, and re-reading the protocols, and crossing your fingers that you don’t kill the cells, you gingerly hold that vial in a waterbath like it is a rare diamond. In […]
By Jessica Hartman, Ph.D. Toiling in the lab With a deadline on its way To the scope I go On a Saturday I don’t see a clone I’ve looked for one that’s right What misery these data bring, I’ll have to look all night, Oh! Single cells, single cells, dilutions aren’t the way Oh, I […]
Cell line development is a key tool and requirement in almost every experimental workflow in drug discovery and development. The ability to track and trace single cells through clonal development is a critical need, not only for research scientists but also for audit trails and regulatory filings. Several tools exist for this process, from simple […]
Monoclonal cell lines are a powerful tool for biomedical research and drug development. The development and use of monoclonal cell lines have been accelerated due to the advent of breakthrough gene editing technologies such as CRISPR-Cas9. Due to their ease of use and amenability to single cell cloning, there are several well-established and recognizable cell […]
Physiological relevance versus reproducibility — As scientists, we all recognize the limitations of developing assays using 2D immortalized cell lines and thus have dedicated our careers to developing cellular models that more closely recapitulate in vivo biology. The reality is, with each step we take down the path of complexity—primary cells, co-culture, 3D models—we also […]
