Papers & Publications

Publications Using the CellRaft™ Technology

See these scientific publications for demonstrations of the various uses for products based on the CellRaft Technology.

Wang Y, Sims CE, Allbritton NL
Lab Chip. 2012 Sep 7;12(17):3036-9. doi: 10.1039/c2lc40330g. Epub 2012 Jul 20

The widespread use of lab-on-a-chip devices containing microcavities in the form of microwells, traps, dead ends, and corners, is often precluded by the trapping of air bubbles. Allbritton and colleagues present a straightforward and simple approach to mitigating bubble formation that can be applied to the CytoSort™ Arrays that are the cornerstone of the CellRaft® Technology. Air bubbles are eliminated in a two-step process, whereby microarrays are first hydrophilized by plasma treatment, and subsequently coated with a monosaccharide such as D-glucose or D-sorbitol. Following this treatment, microwell arrays can be stored for as long as 6 months in air, and complete rewetting of the microwells is demonstrated by the dissolution of the monosaccharide with an aqueous solution.

Wang Y, Phillips C, Xu W, Pai JH, Dhopeshwarkar R, Sims CE, Allbritton NL
Lab Chip. 2010 Nov 7;10(21):2917-24. doi: 10.1039/c0lc00186d. Epub 2010 Sep 13

Selection and isolation of single cells from a mixed population is a common procedure, for example, during the development of clonal cell lines that are genetically engineered, derived from stem cells, or grown from patient samples, single cells must be isolated and then cloned to form a homogeneous population. While myriad methods exist, they commonly rely on enzymatic or mechanical release, which imposes drawbacks in terms of loss of cell morphology, removal of cell surface markers, damage to cell membranes, alterations in cellular physiology, and loss of viability. In this seminal work describing the CellRaft® Technology, the authors demonstrate the fabrication of a microwell array that possesses detachable concave elements, termed CellRafts®, using a PDMS mold combined with standard dip-coating procedures. The resulting microarrays have low auto-fluorescence and are easily removable, in a targeted fashion, allowing for isolation and clonal expansion of single cells. Furthermore, the CytoSort™ Arrays allow for the assessment of cells based on morphology in a time-resolved manner and can be tailored to workflows requiring few to hundreds of thousands of cells.

Wierman M.B., Burbulis I.E., Chronister W.D., Bekiranov S., McConnell M.J.
In: Frade J., Gage F. (eds) Genomic Mosaicism in Neurons and Other Cell Types. Neuromethods, vol 131. Humana Press, New York, NY (2017). doi.org/10.1007/978-1-4939-7280-7_6

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