RaftNotes

RaftNotes are applications and protocols for use with the CellRaft Technology

These publications cover materials and methods, tips, tricks, examples, and unique array formats that help our users achieve their best research results.

Gene editing workflows require transformation of a large number of cells, followed by isolation of individual cells from the larger population to establish clonal colonies. Given the large number of gene edits required for contemporary research and the labor-intensive components of the workflow, there is an unmet need to automate post-transformation cloning.

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Research involving non-adherent cells such as lymphocytes and other primary blood cells is immensely important for applications including drug discovery, cell therapy, and immunology. Non-adherent cells are also of interest in understanding the activity of the Cas9 nuclease, as transfection of these cells is highly variable and inefficient. To enable the use of the CytoSort Array with non-adherent cell lines, we investigated seven common surface coatings and developed recommended protocols.

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Single cell genomic analysis has already impacted a range of life science fields, including tumor clonality, neuronal mosaicism, and drug resistance mechanisms. The CellRaft Technology uniquely enables the sorting and isolation of single nuclei for downstream analysis. Here we provide optimal methods for imaging nuclei on CytoSort Arrays, and downstream molecular biology protocols for the amplification and preparation of nucleic acid prior to sequencing.

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Sorting and isolating single cells is a key sample preparation step in many contemporary workflows including genome editing by CRISPR/Cas9, single cell genomics and differentiation of induced pluripotent stem cells (IPSCs) along with a broad range of other methods. Here, we present optimized methods for staining live cells on the Cell Microsystems CytoSort Array, a microwell array with releasable single cell isolation features called CellRafts.

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