RaftNotes

RaftNotes describe applications and protocols for use with the CellRaft® Technology

These publications cover materials and methods, tips, tricks, examples, and unique array formats that help our users achieve their best research results.

Research involving non-adherent cells such as lymphocytes and other primary blood cells is immensely important for applications including drug discovery, cell therapy, and immunology. Non-adherent cells are also of interest in understanding the activity of the Cas9 nuclease, as transfection of these cells is highly variable and inefficient. To enable the use of the CytoSort® Array with non-adherent cell lines, we investigated seven common surface coatings and developed recommended protocols.

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Sorting and isolating single cells is a key sample preparation step in many contemporary workflows including genome editing by CRISPR/Cas9, single cell genomics and differentiation of induced pluripotent stem cells (IPSCs) along with a broad range of other methods. Here, we present optimized methods for staining live cells on the Cell Microsystems CytoSort® Array, a microwell array with releasable single cell isolation features called CellRafts.

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