Everyday, scientific and medical researchers use the CellRaft AIR system to better understand biology at the single cell level and accelerate advances in discovery
CRISPR
Automating the CRISPR workflow allows for improvement in cell viability, clonality and efficiency so dozens of cell lines can be generated in parallel. Learn more about how our CellRaft AIR System and CytoSort arrays contribute to a reduction in the entire workflow to less than one week for dozens of cell lines, compared to months for just one.
Stem Cells
Working with stem cells presents unique and frequent challenges – particularly with induced pluripotent stem cells and human embryonic stem cells. Generating cell lines, directing differentiation, and reprogramming are all areas that require a gentle approach to capture single cells and culture them under prescribed conditions. The CellRaft AIR System provides an approach to gently isolate single iPS cells or hES cells in distinct microwells on a CellRaft Array.
Genomics
The CellRaft AIR System integrates single cell imaging and viable cell isolation, with no minimum input requirement – and works with virtually any cell type. Learn more about the CellRaft AIR system and how it can accelerate your single cell analysis research.
Organoids
Working with stem cells presents unique and frequent challenges – particularly with induced pluripotent stem cells and human embryonic stem cells. Generating cell lines, directing differentiation, and reprogramming are all areas that require a gentle approach to capture single cells and culture them under prescribed conditions. The CellRaft AIR System provides an approach to gently isolate single iPSCs or hESCs in distinct microwells on a CellRaft Array.
Â
LEARN MORE ABOUT PRODUCT APPLICATIONS IN OUR RAFTNOTES
Making the Impossible Possible: Platform and Protocols to Develop Clonal iPSC Derived Organoids
In the last decade, several protocols and commercial kits have been launched to help differentiate iPSCs into multicellular, neuronal organoids that closely resemble human brain development, including region-specific cellular composition and functional physiology. However, the adoption of these organoid models is still limited to relatively low throughout applications, as the workflows are hampered by challenges in reproducibility and scalability, as well as being manually intensive. Here we report the use of the CellRaft® Technology, to develop and enable streamlined, reproducible organoid workflows that offer reliable imaging, software-guided selection, and automated isolation of single organoids for downstream applications.
Taking the fear out of iPS cell line development
Despite significant strides in technology and increased access for researchers, iPSC culture remains a challenge, thereby limiting its widespread utility for therapeutic and research use. Common pain points include:
1) iPSC lines are sensitive and easily perturbed, requiring constant maintenance.
2) Poor culture conditions and cell line instability can lead to spontaneous differentiation and loss of pluripotency.
3) Generating monoclonal cell lines is incredibly challenging, with low efficiency and lack of proven clonality.
4) The labor, cost, and reagent burden associated with iPSC maintenance and workflows are incredibly high and often prohibitive for the end user.
Download this RaftNote to see how some of these challenges can be overcome.
Image, Identify, and Isolate Single Organoids using the CellRaft AIR® System
The advancement of 3D culture systems has transformed cell-based assays for biological research and drug discovery due to their ability to re-capitulate the structure and cellular complexity of in vivo tissues. Organoids are unique due in their ability to self-organize and closely replicate in vivo pathophysiology. Most laboratory instruments employed to assess endpoints using traditional organoid cultures have limitations that preclude assessment of the heterogeneity within the population or retrieval of single, intact 3D structures for downstream applications.
