Applications

Everyday, scientific and medical researchers use the CellRaft AIR system to better understand biology at the single cell level and accelerate advances in discovery

CRISPR

Automating the CRISPR workflow allows for improvement in cell viability, clonality and efficiency so dozens of cell lines can be generated in parallel. Learn more about how our CellRaft AIR System and CytoSort arrays contribute to a reduction in the entire workflow to less than one week for dozens of cell lines, compared to months for just one.

Stem Cells

Working with stem cells presents unique and frequent challenges – particularly with induced pluripotent stem cells and human embryonic stem cells. Generating cell lines, directing differentiation, and reprogramming are all areas that require a gentle approach to capture single cells and culture them under prescribed conditions. The CellRaft AIR System provides an approach to gently isolate single iPS cells or hES cells in distinct microwells on a CytoSort Array.

Genomics

The CellRaft AIR System integrates single cell imaging and viable cell isolation, with no minimum input requirement – and works with virtually any cell type. Learn more about the CellRaft AIR system and how it can accelerate your single cell analysis research.

Organoids

Working with stem cells presents unique and frequent challenges – particularly with induced pluripotent stem cells and human embryonic stem cells. Generating cell lines, directing differentiation, and reprogramming are all areas that require a gentle approach to capture single cells and culture them under prescribed conditions. The CellRaft AIR System provides an approach to gently isolate single iPSCs or hESCs in distinct microwells on a CytoSort Array.

 

LEARN MORE ABOUT PRODUCT APPLICATIONS IN OUR RAFTNOTES

Image, Identify, and Isolate Single Organoids using the CellRaft AIR® System

The advancement of 3D culture systems has transformed cell-based assays for biological research and drug discovery due to their ability to re-capitulate the structure and cellular complexity of in vivo tissues. Organoids are unique due in their ability to self-organize and closely replicate in vivo pathophysiology. Most laboratory instruments employed to assess endpoints using traditional organoid cultures have limitations that preclude assessment of the heterogeneity within the population or retrieval of single, intact 3D structures for downstream applications.

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Using the CellRaft AIR® System to Image, Identify, and Isolate Suspension Cells

The AIR System offers unique advantages over technologies, such as flow cytometry and droplet dispensers, designed to assess cells in suspension. The limitations to other technologies include impacts on cell viability, inability to interrogate small numbers of cells, and reliance on fluorescent markers or staining for cell characterization. To demonstrate the value of the CellRaft technology for use in suspension cell line development, we have established methodologies for attaching suspension cells to the CytoSort Arrays during single cell expansion while still allowing the cells to expand in suspension after the clone of interest has been isolated.

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Cell Line Development using the CellRaft AIR® System

Therapeutic proteins play an essential role in the biological pharmaceutical market and are used in the treatment of many diseases, such as diabetes, cancer, and anemia. One of the main goals in recombinant protein development is the establishment of high-quality monoclonal cell lines that consistently express large amounts of the given protein. Chinese hamster ovary (CHO) cell lines have dominated the industry as commercial hosts for recombinant protein production; however, the process of generating a homogenous CHO cell line is not trivial.

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